Abstract |
This study evaluated the genetic diversity of multi- drug resistant Campylobacter jejuni (n=44) and C. coli ( n=30) isolated from 18 turkey houses. Antimicrobial resistances to ampicillin, ciprofloxacin and nalidixic acid were higher (P<0.05) in C. coli than in C. jejuni strains. PCR analysis indicated that 82% of total isolates tested, including 91% of C. jejuni and 70% of C. coli tested positive for a 496-bp UDP-glucose 4-epimerase (galE) gene. The diversity of isolates was mapped by antibiogram, SmaI-PFGE and flaA-RFLP typing methods using the discriminatory index (DI). RFLP was more suitable in discriminating C. coli (DI=0.895) than PFGE (DI=0.816) or antibiogram profile (DI=0.552), while either PFGE (DI=0.941) or RFLP (DI=0.942) could be used in discriminating C. jejuni strains. The combined PFGE and antibiogram dendrogram had the highest DI for both C. coli (0.910) and C. jejuni (0.968), suggesting that a combination of typing methods is more useful in examining the diverse Campylobacter population on turkey farms.
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Authors | Rajesh Nayak, Tabitha Stewart, Mohamed Nawaz, Carl Cerniglia |
Journal | Food microbiology
(Food Microbiol)
Vol. 23
Issue 4
Pg. 379-92
(Jun 2006)
ISSN: 0740-0020 [Print] England |
PMID | 16943028
(Publication Type: Journal Article)
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Chemical References |
- Anti-Bacterial Agents
- DNA, Bacterial
- UDPglucose 4-Epimerase
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Topics |
- Animals
- Anti-Bacterial Agents
(pharmacology)
- Campylobacter coli
(drug effects, genetics, isolation & purification)
- Campylobacter jejuni
(drug effects, genetics, isolation & purification)
- DNA, Bacterial
(analysis)
- Drug Resistance, Bacterial
- Electrophoresis, Gel, Pulsed-Field
- Food-Processing Industry
(standards)
- Genetic Variation
- Genotype
- Humans
- Microbial Sensitivity Tests
- Polymerase Chain Reaction
(methods)
- Polymorphism, Restriction Fragment Length
- Prevalence
- Species Specificity
- Turkeys
(microbiology)
- UDPglucose 4-Epimerase
(genetics)
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