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In vitro antimicrobial susceptibility, genetic diversity and prevalence of UDP-glucose 4-epimerase (galE) gene in Campylobacter coli and Campylobacter jejuni from Turkey production facilities.

Abstract
This study evaluated the genetic diversity of multi-drug resistant Campylobacter jejuni (n=44) and C. coli (n=30) isolated from 18 turkey houses. Antimicrobial resistances to ampicillin, ciprofloxacin and nalidixic acid were higher (P<0.05) in C. coli than in C. jejuni strains. PCR analysis indicated that 82% of total isolates tested, including 91% of C. jejuni and 70% of C. coli tested positive for a 496-bp UDP-glucose 4-epimerase (galE) gene. The diversity of isolates was mapped by antibiogram, SmaI-PFGE and flaA-RFLP typing methods using the discriminatory index (DI). RFLP was more suitable in discriminating C. coli (DI=0.895) than PFGE (DI=0.816) or antibiogram profile (DI=0.552), while either PFGE (DI=0.941) or RFLP (DI=0.942) could be used in discriminating C. jejuni strains. The combined PFGE and antibiogram dendrogram had the highest DI for both C. coli (0.910) and C. jejuni (0.968), suggesting that a combination of typing methods is more useful in examining the diverse Campylobacter population on turkey farms.
AuthorsRajesh Nayak, Tabitha Stewart, Mohamed Nawaz, Carl Cerniglia
JournalFood microbiology (Food Microbiol) Vol. 23 Issue 4 Pg. 379-92 (Jun 2006) ISSN: 0740-0020 [Print] England
PMID16943028 (Publication Type: Journal Article)
Chemical References
  • Anti-Bacterial Agents
  • DNA, Bacterial
  • UDPglucose 4-Epimerase
Topics
  • Animals
  • Anti-Bacterial Agents (pharmacology)
  • Campylobacter coli (drug effects, genetics, isolation & purification)
  • Campylobacter jejuni (drug effects, genetics, isolation & purification)
  • DNA, Bacterial (analysis)
  • Drug Resistance, Bacterial
  • Electrophoresis, Gel, Pulsed-Field
  • Food-Processing Industry (standards)
  • Genetic Variation
  • Genotype
  • Humans
  • Microbial Sensitivity Tests
  • Polymerase Chain Reaction (methods)
  • Polymorphism, Restriction Fragment Length
  • Prevalence
  • Species Specificity
  • Turkeys (microbiology)
  • UDPglucose 4-Epimerase (genetics)

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