Abstract | BACKGROUND: METHODS: Sixteen COPD subjects participated in the study. Theophylline (400 mg/day orally) or FP (400 mug/day inhalation) were administered for 4 weeks in a randomised crossover manner with a washout period of 4 weeks. Induced sputum was collected at the beginning and end of each treatment period. 3-nitrotyrosine (3-NT), which is a footprint of RNS, was quantified by high performance liquid chromatography with an electrochemical detection method as well as by immunohistochemical staining. RESULTS:
Theophylline significantly reduced the level of 3-NT in the sputum supernatant as well as the number of 3-NT positive cells (both p<0.01). FP also reduced 3-NT formation, but the effect was smaller than that of theophylline. Theophylline also significantly reduced the neutrophil cell counts in the sputum (p<0.01), while FP treatment had no effect on the number of inflammatory cells in the sputum, except eosinophils. CONCLUSIONS:
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Authors | T Hirano, T Yamagata, M Gohda, Y Yamagata, T Ichikawa, S Yanagisawa, K Ueshima, K Akamatsu, M Nakanishi, K Matsunaga, Y Minakata, M Ichinose |
Journal | Thorax
(Thorax)
Vol. 61
Issue 9
Pg. 761-6
(Sep 2006)
ISSN: 0040-6376 [Print] England |
PMID | 16936236
(Publication Type: Comparative Study, Journal Article, Randomized Controlled Trial)
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Chemical References |
- Adrenal Cortex Hormones
- Androstadienes
- Bronchodilator Agents
- Reactive Nitrogen Species
- Theophylline
- Fluticasone
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Topics |
- Administration, Inhalation
- Administration, Oral
- Adrenal Cortex Hormones
(administration & dosage)
- Aged
- Androstadienes
(administration & dosage)
- Bronchodilator Agents
(administration & dosage)
- Cross-Over Studies
- Female
- Fluticasone
- Forced Expiratory Volume
(drug effects)
- Humans
- Male
- Oxidative Stress
- Pulmonary Disease, Chronic Obstructive
(drug therapy, metabolism, physiopathology)
- Reactive Nitrogen Species
(biosynthesis)
- Theophylline
(administration & dosage)
- Vital Capacity
(drug effects)
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