HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Isolation and characterization of Borna disease agent cDNA clones.

Abstract
Borna disease (BD) is a neurologic syndrome characterized by behavioral disturbances and the accumulation of specific proteins in limbic system neurons. A viral etiology has been proposed because BD can be induced in birds, rodents, and primates by inoculation with filtered brain homogenates from animals with BD. We report here the isolation and preliminary characterization of cDNA clones from a rat with BD. These clones hybridized to specific transcripts in BD rat brain and arrested in vitro translation of BD proteins. In situ hybridization experiments using RNA probes prepared from these clones showed an abundance of these transcripts in limbic system neurons. Northern (RNA) hybridizations using these RNA probes indicated that the BD agent is probably a virus with major transcripts of 8.5, 2.1, and 0.8 kilobases.
AuthorsW I Lipkin, G H Travis, K M Carbone, M C Wilson
JournalProceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 87 Issue 11 Pg. 4184-8 (Jun 1990) ISSN: 0027-8424 [Print] United States
PMID1693432 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • RNA Probes
  • RNA, Messenger
  • Viral Proteins
  • Poly A
  • RNA
  • DNA
Topics
  • Animals
  • Borna Disease (microbiology)
  • Borna disease virus (genetics)
  • Brain (microbiology)
  • Cloning, Molecular
  • DNA (genetics)
  • Limbic System (microbiology)
  • Molecular Weight
  • Nucleic Acid Hybridization
  • Poly A (genetics)
  • Precipitin Tests
  • RNA (genetics)
  • RNA Probes
  • RNA, Messenger (genetics)
  • Rats
  • Viral Proteins (genetics)
  • Viruses, Unclassified (genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: