HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Testosterone increases TSH-beta mRNA, and modulates alpha-subunit mRNA differentially in mouse thyrotropic tumor and castrate rat pituitary.

Abstract
TSH, LH and FSH, the three pituitary glycoprotein hormones, are each composed of a common alpha-subunit and a hormone specific beta-subunit. Testosterone is known to regulate all three intact hormones differently in the rodent. However, there is only one gene encoding the common alpha-subunit. In order to elucidate the effects of testosterone on TSH subunit synthesis and its regulation of the common alpha-subunit, two in vivo models were studied: castrate rat pituitary was used as a gonadotropin-enriched tissue; and mouse thyrotropic tumor was used as a thyrotropin-enriched tissue. Male castrate rats were treated with testosterone propionate, 500 micrograms/100 g BW, sc, for 11 days. Testosterone increased plasma TSH to 131% of control values (P less than 0.02), while plasma LH fell to undetectable levels, and plasma alpha-subunit fell to 14% of control values (P less than 0.001). Testosterone increased TSH-beta mRNA to 237% of control values (P less than 0.02), while alpha-subunit mRNA fell to 20% of control values (P less than 0.001). Hypothyroid mice bearing thyrotropic tumors were treated with testosterone propionate, 150 micrograms/100 g BW, sc, for 11 days. In this model plasma TSH-beta and alpha-subunit concentrations are 1000-fold higher than in non-tumor bearing animals, and the contribution of pituitary gonadotropes to plasma subunit concentrations is negligible. "Total" TSH-beta and alpha-subunit concentrations were estimated as one-half of intact TSH plus the respective free subunit concentration.(ABSTRACT TRUNCATED AT 250 WORDS)
AuthorsD S Ross
JournalHormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et meĢtabolisme (Horm Metab Res) Vol. 22 Issue 3 Pg. 163-9 (Mar 1990) ISSN: 0018-5043 [Print] GERMANY, WEST
PMID1693130 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Glycoprotein Hormones, alpha Subunit
  • Glycoproteins
  • Gonadotropins
  • RNA, Messenger
  • Testosterone
  • Thyrotropin
Topics
  • Animals
  • Gene Expression
  • Glycoprotein Hormones, alpha Subunit (genetics)
  • Glycoproteins (metabolism)
  • Gonadotropins (genetics)
  • Male
  • Mice
  • Orchiectomy
  • Pituitary Gland (metabolism)
  • RNA, Messenger (genetics)
  • Rats
  • Testosterone (genetics)
  • Thyroid Neoplasms (genetics)
  • Thyrotropin (biosynthesis, blood, genetics)
  • Time Factors

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: