Expression of beta-defensins in the tubotympanum of experimental otitis media.

Since the expression levels of beta-defensins 2-4 were up-regulated in experimental otitis media, they may play a protective role in the pathogenesis of otitis media.
Defensins are antimicrobial peptides that play a major role in innate immunity. The goal of this study was to identify the expression of defensins in experimental otitis media of the mouse.
The expression of three alpha-defensins (cryptdins, cryptdin-related sequences 1-C (CRS1-C), and CRS4-C) and four beta-defensins (mBD1, mBD2, mBD3, and mBD4) was investigated in the tubotympanum of experimental otitis media in mice by a semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR), and the expression levels of three beta-defensins (mBD2, mBD3, mBD4) were evaluated by Western blotting.
The alpha-defensins were not expressed in the tubotympanum. mBD1 was expressed constitutively in normal middle ear mucosa and Eustachian tube mucosa, but up-regulated expression of mBD2, mBD3, and mBD4 was observed with RT-PCR and Western blotting in the tubotympanums in experimental otitis media, while the normal tubotympanums did not express them.
AuthorsDong Jin Shin, Semeibsuren Gan-Undram, Seo Jin Kim, Young Joon Jun, Gi Jung Im, Hak Hyun Jung
JournalActa oto-laryngologica (Acta Otolaryngol) Vol. 126 Issue 10 Pg. 1040-5 (Oct 2006) ISSN: 0001-6489 [Print] Norway
PMID16923707 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Endotoxins
  • RNA, Messenger
  • beta-Defensins
  • Animals
  • Blotting, Western
  • Disease Models, Animal
  • Ear, Middle (immunology, metabolism)
  • Endotoxins (toxicity)
  • Eustachian Tube (immunology, metabolism)
  • Gene Expression
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Otitis Media (chemically induced, immunology, metabolism)
  • RNA, Messenger (biosynthesis)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Analysis, Protein
  • Up-Regulation
  • beta-Defensins (analysis, biosynthesis, genetics)

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