[Asparagine synthetase activity in pediatric acute lymphoblastic leukemia].

Abstract

OBJECTIVE:

To study the cellular activity of asparagine synthetase in different types of childhood acute lymphoblastic leukemia (ALL).

METHODS:

The cellular activity of asparagine synthetase was detected by HPLC-FLD and Protein measurement in 28 ALL children (7 cases of T-ALL and 21 cases of B-lymphoid lineage ALL) before chemotherapy.

RESULTS:

The asparagines synthetase activity levels in T-ALL children were significantly higher than those of the B-lymphoid lineage ALL patients, with the median activity level of 9.3 nM Asn/mg protein/hr vs 5.2 nM Asn/mg protein/hr (P < 0.05). The distribution of the asparagine synthetase activity demonstrated a polymorphism in either T-ALL or B-lymphoid lineage ALL patients.

CONCLUSIONS:

The cellular activity of asparagines synthetase in ALL patients is presented with a polymorphism distribution. The asparagines synthetase activity levels in T-ALL are significantly higher than in B-lymphoid lineage ALL.

• Authors: Hua Jiang, Long-Jun Gu, Hui-Liang Xue, Jing-Yan Tang, Jing Chen, Ci Pan, Jing Chen
• Journal: Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics (Zhongguo Dang Dai Er Ke Za Zhi) Vol. 8 Issue 4 Pg. 272-4 (Aug 2006) ISSN: 1008-8830 [Print] China
• PMID: 16923354 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Core Binding Factor Alpha 2 Subunit
• Oncogene Proteins, Fusion
• RNA, Messenger
• TEL-AML1 fusion protein
• Asparaginase
• Aspartate-Ammonia Ligase

Topics

• Asparaginase (therapeutic use)
• Aspartate-Ammonia Ligase (genetics, metabolism)
• Child
• Core Binding Factor Alpha 2 Subunit (genetics)
• Female
• Humans
• Male
• Oncogene Proteins, Fusion (genetics)
• Precursor Cell Lymphoblastic Leukemia-Lymphoma (drug therapy, enzymology)
• RNA, Messenger (analysis)