Abstract |
Methylchloroisothiazolinone and methylisothiazolinone (MCI/MI) contact allergy affects 1-3% of patch-tested patients in European centres. The aim of the present study was to evaluate the importance of the factors--time and concentration (dose/per unit area)--in the elicitation capacity by means of a repeated open application test (ROAT) experimental design. The study was designed as a double-blind, placebo-controlled, dose-response ROAT preceded by a diagnostic patch testing. 25 subjects with confirmed MCI/MI allergy and 10 healthy, non-MCI/MI allergic control subjects were exposed to 0.025 microg/cm2 (2 p.p.m.) of MCI/MI/unit area of the skin for 4 weeks. After a wash-out period of at least 4 weeks, the subjects were exposed to 0.094 microg/cm2 (7.5 p.p.m.) of MCI/MI/unit area of the skin for 4 weeks. The study showed the importance of both time and exposure in the elicitation process. It demonstrated that the elicitation threshold for MCI/MI is expected to be in the proximity of 0.025 microg/cm2 although it was not possible to establish a definitive elicitation threshold for MCI/MI in this study.
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Authors | Claus Zachariae, Anne Lerbaek, Pauline M McNamee, John E Gray, Mike Wooder, Torkil Menné |
Journal | Contact dermatitis
(Contact Dermatitis)
Vol. 55
Issue 3
Pg. 160-6
(Sep 2006)
ISSN: 0105-1873 [Print] England |
PMID | 16918615
(Publication Type: Journal Article, Randomized Controlled Trial)
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Chemical References |
- Allergens
- Preservatives, Pharmaceutical
- Thiazoles
- 2-methyl-4-isothiazolin-3-one
- 5-chloro-2-methyl-4-isothiazolin-3-one
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Topics |
- Adolescent
- Adult
- Allergens
(adverse effects)
- Dermatitis, Allergic Contact
(diagnosis, etiology, pathology)
- Dose-Response Relationship, Drug
- Double-Blind Method
- Female
- Humans
- Male
- Patch Tests
(standards)
- Preservatives, Pharmaceutical
(adverse effects)
- Thiazoles
(adverse effects)
- Time Factors
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