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Rapid detection of Pneumocystis carinii in bronchoalveolar lavage samples by using Cellufluor staining.

Abstract
Cellufluor (Calcofluor white) has been found to be a useful, rapid chemofluorescent stain for detection of Pneumocystis carinii cysts in bronchoalveolar lavage samples. When compared with toluidine blue O and Giemsa stains on 45 specimens (22 positive and 23 negative), the sensitivity and specificity of the Cellufluor stain were 95 and 100%, respectively.
AuthorsV S Baselski, M K Robison, L W Pifer, D R Woods
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 28 Issue 2 Pg. 393-4 (Feb 1990) ISSN: 0095-1137 [Print] United States
PMID1690218 (Publication Type: Journal Article)
Chemical References
  • Fluorescent Dyes
  • Organic Chemicals
  • cellufluor
Topics
  • Bronchoalveolar Lavage Fluid (microbiology)
  • Fluorescent Dyes
  • Humans
  • Organic Chemicals
  • Pneumocystis (isolation & purification)
  • Pneumonia, Pneumocystis (diagnosis, microbiology)
  • Staining and Labeling

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