Allergenic contribution of the IgE-reactive domains of the 1,3-beta-glucanase Ole e 9: diagnostic value in olive pollen allergy.

Abstract

BACKGROUND:

Designing of methods for an accurate diagnosis is a main goal of allergy research. Olive pollen allergy is currently diagnosed using commercially available pollen extracts that do not allow identification of the molecules that elicit the disease.

OBJECTIVE:

To analyze the suitability of using the N- and C-terminal domains (NtD and CtD, respectively) of the 1,3-beta-glucanase Ole e 9, a major allergen from olive pollen, for in vitro diagnosis.

METHODS:

Serum samples from 55 olive-allergic patients were assayed using enzyme-linked immunosorbent assay to study hypersensitive patients with IgE reactivity to Ole e 9. The specific IgEs to NtD and CtD, obtained by recombinant technology, were determined by means of immunoblotting, enzyme-linked immunosorbent assay, and inhibition assays.

RESULTS:

Thirty-one of 33 serum samples from Ole e 9-allergic patients were IgE reactive to recombinant NtD (rNtD) (n = 26 [79%]), recombinant CtD (rCtD) (n = 22 [67%]), or both (n = 17 [52%]). Nine patients (27%) were exclusively reactive to rNtD and 5 (15%) to rCtD. Inhibition assays of IgE binding to Ole e 9 with a mixture of both domains abolished 90% of the binding, whereas 44% and 45% were abolished when rNtD and rCtD were used, respectively.

CONCLUSIONS:

Because sensitization to NtD or CtD of Ole e 9 could be correlated to vegetable food-latex-pollen cross-reactivity processes or to the exacerbation and persistence of asthma, respectively, these molecules could be used in vitro as markers of disease to classify patients and to design a patient-tailored immunotherapy approach.

• Authors: Oscar Palomares, Mayte Villalba, Joaquín Quiralte, Rosalía Rodríguez
• Journal: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology (Ann Allergy Asthma Immunol) Vol. 97 Issue 1 Pg. 61-5 (Jul 2006) ISSN: 1081-1206 [Print] United States
• PMID: 16892783 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Antigens, Plant
• Peptide Fragments
• Plant Proteins
• Recombinant Fusion Proteins
• Immunoglobulin E
• OLE9 protein, Olea europaea
• beta-Glucosidase

Topics

• Adolescent
• Adult
• Allergens (chemistry, genetics, immunology)
• Antigen-Antibody Reactions
• Antigens, Plant
• Asthma (diagnosis, etiology, immunology)
• Binding, Competitive
• Child
• Enzyme-Linked Immunosorbent Assay
• Female
• Humans
• Immunoblotting
• Immunoglobulin E (blood, immunology)
• Male
• Middle Aged
• Olea (immunology)
• Peptide Fragments (immunology)
• Plant Proteins (chemistry, genetics, immunology)
• Pollen (adverse effects, immunology)
• Protein Structure, Tertiary
• Recombinant Fusion Proteins (immunology)
• Rhinitis, Allergic, Seasonal (diagnosis, etiology, immunology)
• Skin Tests
• beta-Glucosidase (chemistry, genetics, immunology)