Abstract |
We previously reported that inoculation of rats with live vaccinia virus (VV) recombinants VVpyMT, VVpyLT expressing either the middle-T (MT) or large-T (LT) proteins of polyomavirus (PyV) can elicit immunity to challenge with syngeneic PyV- tumor cells. We now report the results of cross-vaccination studies. VVpyMT was ineffective against cells expressing LT protein but prevented development of MT-expressing cells. Conversely, the VVpyLT was ineffective against MT-expressing cells. In the two experiments performed, tumor growth enhancement rather than retardation was observed in VVpyLT-vaccinated animals receiving PyV-LT (FRLTI) challenge tumor cells. To determine the location of the major TSTA within MT, a further VV recombinant (VVpyMT/Cfr) was constructed that expresses only the unique C-terminal segment of MT. VVpyMT-Cfr and VVpyMT were equally effective in eliciting tumor immunity, indicating the presence of a major TSTA epitope within the unique C-terminal region of MT.
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Authors | M P Kieny, C Gautier, C Tomasetto, I Kuhn, M Hareuveni, P Clertant, R Lathe |
Journal | International journal of cancer
(Int J Cancer)
Vol. 45
Issue 1
Pg. 185-9
(Jan 15 1990)
ISSN: 0020-7136 [Print] United States |
PMID | 1688831
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Polyomavirus Transforming
- Epitopes
- Recombinant Proteins
- Viral Vaccines
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Topics |
- Amino Acid Sequence
- Animals
- Antigens, Polyomavirus Transforming
(genetics, immunology)
- Base Sequence
- Cross Reactions
(immunology)
- Epitopes
(genetics, immunology)
- Female
- Immunization
- Molecular Sequence Data
- Polyomavirus
(genetics, immunology)
- Rats
- Rats, Inbred F344
- Recombinant Proteins
(immunology)
- Transcription, Genetic
(genetics)
- Tumor Virus Infections
(immunology, prevention & control)
- Vaccinia virus
(immunology)
- Viral Vaccines
(immunology)
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