Characterization of the heparin binding site in the N-terminus of human pro-islet amyloid polypeptide: implications for amyloid formation.

Abstract

Islet amyloid deposits are a characteristic pathological hallmark of type 2 diabetes mellitus. Islet amyloid polypeptide (IAPP), also referred to as amylin, aggregates in the islet extracellular space to form amyloid deposits in up to 95% of patients with the disease. IAPP is stored with insulin in beta-islet cells and is processed in parallel by subtilisin-like prohormone convertases prior to secretion. There is indirect evidence that normal processing of the prohormone precursor, proIAPP, at the N-terminal cleavage site is defective in type 2 diabetes and results in secretion of an N-terminal extended proIAPP intermediate. The N-terminal flanking region of proIAPP is detected in amyloid deposits; however, the C-terminal flanking region is not. Immunohistochemical studies implicate the presence of the heparan sulfate proteoglycan (HSPG) perlecan in islet amyloid deposits, suggesting a role for HSPGs in mediating amyloid deposition in type 2 diabetes and implicating a binding domain in the N-terminus of proIAPP. Initial studies of proIAPP indicated that the HSPG binding region is contained within the first 30 residues. Here, we characterize the potential HSPG binding site of proIAPP in detail by analyzing a set of peptide fragments. Binding is tighter at low pH due to protonation of histidine residues. Deletion studies show that Arg-22 and His-29 play a role in binding. Reduction of the Cys-13 to Cys-18 disulfide leads to a noticeable decrease in binding. We demonstrate the ability of heparan sulfate to induce amyloid formation in N-terminal fragments of proIAPP. The oxidized peptide forms amyloid more rapidly than the reduced variant in the presence of heparan sulfate, but the reduced peptide ultimately forms more extensive amyloid deposits. The potential implications for islet amyloid formation in vivo are discussed.

• Authors: Andisheh Abedini, Sylvia M Tracz, Jae-Hyun Cho, Daniel P Raleigh
• Journal: Biochemistry (Biochemistry) Vol. 45 Issue 30 Pg. 9228-37 (Aug 01 2006) ISSN: 0006-2960 [Print] United States
• PMID: 16866369 (Publication Type: Comparative Study, Journal Article, Research Support, N.I.H., Extramural, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Amyloid
• Disulfides
• Peptide Fragments
• Protein Precursors
• pro-islet amyloid polypeptide
• Heparin
• Cysteine

Topics

• Amino Acid Sequence
• Amyloid (biosynthesis, chemical synthesis, chemistry, genetics, metabolism)
• Binding Sites (genetics)
• Cysteine (chemistry, metabolism)
• Disulfides (chemistry)
• Heparin (chemistry, genetics, metabolism)
• Humans
• Insulin-Secreting Cells (chemistry, metabolism)
• Molecular Sequence Data
• Peptide Fragments (chemical synthesis, metabolism)
• Protein Precursors (chemical synthesis, metabolism)