Abstract |
Some coumarins possess enhancing effects on lymphocyte mitogen responsiveness. In this investigation, the activity of scopoletin, a coumarin that has been isolated from different plants and in this case specifically from T. cordata Mill., was evaluated. For this purpose, normal T lymphocytes and a hyperproliferative T lymphoma cell line were used. Scopoletin was found to exert a dual action on tumoral lymphocytes exhibiting both a cytostatic and a cytotoxic effect. These effects varied with the concentrations analysed and the time of cell incubation (EC(50): 251+/-15 microg/ml) and were associated to the induction of apoptosis. Scopoletin induced cell proliferation on normal T lymphocytes (Proliferation stimulation index: 1 microg/ml scopoletin: 1.26+/-0.1; 10 microg/ml scopoletin: 3+/-0.25; 100 microg/ml scopoletin: 1.86+/-0.08); this stimulatory action was found to be due to the interaction with kinase C (PKC) protein. These results indicate that scopoletin could be a potential antitumoral compound to be used for cancer treatment.
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Authors | María Gabriela Manuele, Graciela Ferraro, Maria Laura Barreiro Arcos, Paula López, Graciela Cremaschi, Claudia Anesini |
Journal | Life sciences
(Life Sci)
Vol. 79
Issue 21
Pg. 2043-8
(Oct 19 2006)
ISSN: 0024-3205 [Print] Netherlands |
PMID | 16860346
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Immunologic Factors
- Concanavalin A
- Scopoletin
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Topics |
- Animals
- Apoptosis
(drug effects, immunology)
- Cell Line, Tumor
- Cell Proliferation
(drug effects)
- Cell Survival
(drug effects, immunology)
- Concanavalin A
(pharmacology)
- Dose-Response Relationship, Drug
- Flowers
(chemistry)
- Immunologic Factors
(isolation & purification, pharmacology)
- Male
- Mice
- Mice, Inbred C3H
- Scopoletin
(isolation & purification, pharmacology)
- T-Lymphocytes
(drug effects, immunology)
- Tilia
(chemistry)
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