| Abstract | Yorkshire, red Duroc, and F1 (first-generation cross) pigs heal with normal, fibroproliferative/hypercontractile, and intermediate levels of scarring, respectively. The purpose of this study was to evaluate the healing phenotype of Yorkshire x F1 backcross animals, to address the molecular basis for genetic transmission of the red Duroc scarring phenotype. Macroscopically and histologically, full-thickness wounds on backcross animals followed the Yorkshire phenotype, with one exception; the backcross wounds exhibited contraction following re-epithelialization. The molecular expression patterns in the backcross animals generally correlated with the macroscopic and histologic findings. Compared to Yorkshire, red Duroc, and F1 wounds, the backcross wounds demonstrated a diminished initial inflammatory phase, followed by a prolonged expression of several relevant growth factors. Additionally, collagen expression was prolonged, expression of matrix metalloproteinases was increased, and alterations in tissue inhibitor of metalloproteinase expression were detected. Moreover, a subset of molecules still followed the red Duroc pattern of mRNA expression, a finding that allows for correlations between the scarring phenotype and the molecular expression patterns to be made in this model. The results indicate that a number of genes are likely involved in the red Duroc healing phenotype and that identification of the specific genes involved will require a more detailed genomic analysis. |
| Authors | Corrie L Gallant-Behm, Carol Reno, Helen Tsao, David A Hart
(Affiliation: Department of Surgery, University of Calgary, Calgary, Alberta, Canada.)
|
| Journal | The Journal of investigative dermatology
(J Invest Dermatol)
Vol. 127
Issue 1
Pg. 233-44
(Jan 2007)
ISSN: 1523-1747 United States |
| PMID | 16858423
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Cytokines
- RNA, Messenger
- Transforming Growth Factor beta
- Osteopontin
- Fibroblast Growth Factor 7
- Collagen
- Matrix Metalloproteinases
|
| Topics |
- Animals
- Cicatrix
(genetics, pathology)
- Collagen
(genetics)
- Crosses, Genetic
- Cytokines
(genetics)
- Female
- Fibroblast Growth Factor 7
(genetics)
- Gene Expression Profiling
- Matrix Metalloproteinases
(genetics)
- Osteopontin
(genetics)
- RNA, Messenger
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Swine
- Transforming Growth Factor beta
(genetics)
- Wound Healing
(genetics)
|
