[mRNA expression of voltage-dependent potassium channels in the brain of rats after middle cerebral artery occlusion].

Abstract

AIM:

To study the mRNA expression changes in the brain of rats after middle cerebral artery occlusion.

METHODS:

Middle cerebral artery occlusion was used to induce ischemia in rat brain. The mRNA expression of voltage-dependent potassium channel subtypes, including Kv1.4, Kv1.5, Kv2.1 and Kv4.2, were detected in rat hippocampus and cortex by RT-PCR.

RESULTS:

Middle cerebral artery occlusion induced a significant neurological injury in rats. After ischemia 2 h, the mRNA of Kv1.4, Kv2.1 and Kv4.2 in hippocampus increased by 50%, 67% and 90% , respectively. And the mRNA of Kv1.4 and Kv4.2 maintained at a high level in hippocampus after ischemia 24 h. In cortex, the mRNA level of all the four subtypes were not changed significantly after ischemia 2 h, but the mRNA of Kv2.1 and Kv4.2 increased by 70% and 62% after ischemia 24 h, respectively.

CONCLUSION:

The mRNA expression levels of voltage-dependent potassium channels were up-regulated in rat hippocampus and cortex after middle cerebral artery occlusion.

• Authors: Hai-xia Zhang, Zheng-bin Li, Xiao-liang Wang
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 41 Issue 4 Pg. 328-32 (Apr 2006) ISSN: 0513-4870 [Print] China
• PMID: 16856477 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Kv1.4 Potassium Channel
• Kv1.5 Potassium Channel
• Potassium Channels, Voltage-Gated
• RNA, Messenger
• Shab Potassium Channels
• Shal Potassium Channels

Topics

• Animals
• Brain (metabolism)
• Infarction, Middle Cerebral Artery (metabolism)
• Kv1.4 Potassium Channel (biosynthesis, genetics)
• Kv1.5 Potassium Channel (biosynthesis, genetics)
• Male
• Potassium Channels, Voltage-Gated (biosynthesis, genetics)
• RNA, Messenger (biosynthesis, genetics)
• Rats
• Rats, Wistar
• Shab Potassium Channels (biosynthesis, genetics)
• Shal Potassium Channels (biosynthesis, genetics)
• Up-Regulation