Temporal and spatial patterns of transin/stromelysin RNA expression following toxic injury in rat liver.

Abstract	We have examined the expression of the extracellular matrix-degrading metalloprotease transin/stromelysin during the early phases of rat liver regeneration following toxic injury by a single dose of carbon tetrachloride (CCl4). In situ hybridization displayed cell type-specific spatial and temporal RNA expression patterns with high transcript levels in small proportions of hepatocytes and non-parenchymal cells, peaking at 24 and 48 h after intoxication, respectively. In agreement with the presence of c-fos and c-jun recognition sites on the transin gene, expression of these oncogenes preceded transin expression. Transin-expressing hepatocytes were largely localized in areas subsequently eliminated by necrosis due to CCl4 intoxication. As a consequence of these expression patterns and the key function of transin as an activator of interstitial collagenase, it seems that the hepatic fibrosis observed after CCl4 administration may be related to fibrogenesis unbalanced by fibrolysis due to altered transin expression.
Authors	H Herbst, O Heinrichs, D Schuppan, S Milani, H Stein
Journal	Virchows Archiv. B, Cell pathology including molecular pathology (Virchows Arch B Cell Pathol Incl Mol Pathol) Vol. 60 Issue 5 Pg. 295-300 ( 1991) ISSN: 0340-6075 [Print] Germany
PMID	1685036 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Proto-Oncogene Proteins c-fos Proto-Oncogene Proteins c-jun RNA Probes RNA, Messenger Carbon Tetrachloride Metalloendopeptidases Matrix Metalloproteinase 3
Topics	Animals Carbon Tetrachloride (toxicity) Chemical and Drug Induced Liver Injury (enzymology, genetics, pathology) Enzyme Induction Extracellular Matrix (metabolism) Female Fibrosis Liver (metabolism, pathology) Liver Regeneration Matrix Metalloproteinase 3 Metalloendopeptidases (biosynthesis, genetics) Necrosis Nucleic Acid Hybridization Proto-Oncogene Proteins c-fos (biosynthesis, physiology) Proto-Oncogene Proteins c-jun (biosynthesis, physiology) RNA Probes RNA, Messenger (biosynthesis) Rats Rats, Inbred Strains Time Factors Transcription, Genetic

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