[The role of subtypes of voltage-gated K+ channels in pulmonary vasoconstriction induced by 15-hydroeicosatetraenoic acid].

Abstract

AIM:

To observe the effect of subtypes of Kv channels in rat pulmonary artery smooth muscle cells (PASMCs) on the process of pulmonary vasoconstriction induced by 15-HETE.

METHODS:

In the present study, ring of rabbit PA with specific Kv channel blockers were employed to functionally identify certain channel subtypes that took part in the process of 15-HETE induced pulmonary vasoconstriction; RT-PCR and Western blotting analysis were also used to measure the expression of subtypes of Kv in PASMCs exposed to 15-HETE,chronic hypoxia.

RESULTS:

Blocking of Kv1. 1, Kv1. 2, Kv1. 3 and Kv1. 6 channels did not affect 15-HETE induced vasoconstriction in normoxic rats; 15-HETE did not affect expression of Kv1. 1 and Kv1. 2 channels; 15-HETE significantly downregulated the expression of mRNA and protein of Kv1. 5 and Kv2. 1 in rat PASMCs.

CONCLUSION:

The results suggested that hypoxia may block Kv1. 5 and Kv2. 1 channels via 15-HETE mediated mechanism, leading to decrease numbers of functional Kv1. 5 and Kv2. 1 channels in PASMCs, leading to PA vasoconstriction.

• Authors: Qian Li, Rong Zhang, Chang-Lian Lü, Yan Liu, Zhen Wang, Da-Ling Zhu
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 41 Issue 5 Pg. 412-7 (May 2006) ISSN: 0513-4870 [Print] China
• PMID: 16848316 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Hydroxyeicosatetraenoic Acids
• Kv1.5 Potassium Channel
• Potassium Channels, Voltage-Gated
• RNA, Messenger
• Shab Potassium Channels
• 15-hydroxy-5,8,11,13-eicosatetraenoic acid

Topics

• Animals
• Cell Hypoxia
• Cells, Cultured
• Hydroxyeicosatetraenoic Acids (pharmacology)
• Hypoxia (physiopathology)
• Kv1.5 Potassium Channel (biosynthesis, genetics)
• Male
• Muscle, Smooth, Vascular (cytology)
• Myocytes, Smooth Muscle (cytology, metabolism)
• Potassium Channels, Voltage-Gated (antagonists & inhibitors)
• Pulmonary Artery (physiopathology)
• RNA, Messenger (biosynthesis, genetics)
• Rats
• Rats, Wistar
• Shab Potassium Channels (biosynthesis, genetics)
• Vasoconstriction (drug effects)