Atiprimod blocks phosphorylation of JAK-STAT and inhibits proliferation of acute myeloid leukemia (AML) cells.

Abstract

In studies of multiple myeloma cells, atiprimod was shown to block Stat3 activation and inhibited colony-forming cell proliferation. We hypothesized that atiprimod may also inhibit activation of intracellular signaling pathways in AML cells resulting in apoptosis and growth inhibition. We demonstrate that atiprimod inhibited clonogenic growth of AML cell lines and fresh AML marrow cells whereas it did not significantly affect growth of normal hematopoietic progenitors from marrow samples of healthy controls. Atiprimod decreased phosphorylation of Stat3 and Stat5, and protein levels of Jak2, whereas gene expression of Jak2 was not affected. Atiprimod further induced apoptosis by cleavage of caspase 3 and PARP. In summary, our data suggest that atiprimod has a significant antiproliferative and proapoptotic effect on AML cells. This effect may be facilitated by inhibition of the Jak-Stat signaling pathway. Further evaluation of atiprimod in clinical trials of AML should be considered.

• Authors: Stefan Faderl, Alessandra Ferrajoli, David Harris, Quin Van, Hagop M Kantarjian, Zeev Estrov
• Journal: Leukemia research (Leuk Res) Vol. 31 Issue 1 Pg. 91-5 (Jan 2007) ISSN: 0145-2126 [Print] England
• PMID: 16828865 (Publication Type: Journal Article)

Chemical References

• DNA Primers
• Enzyme Inhibitors
• STAT3 Transcription Factor
• STAT5 Transcription Factor
• Spiro Compounds
• azaspirane
• Janus Kinase 2

Topics

• Acute Disease
• Blast Crisis (prevention & control)
• Cell Division (drug effects)
• Cell Line, Tumor
• DNA Primers
• Enzyme Inhibitors (pharmacology)
• Humans
• Janus Kinase 2 (antagonists & inhibitors, genetics)
• Leukemia, Myeloid (genetics, pathology)
• Phosphorylation
• Reverse Transcriptase Polymerase Chain Reaction
• STAT3 Transcription Factor (antagonists & inhibitors)
• STAT5 Transcription Factor (antagonists & inhibitors)
• Spiro Compounds (pharmacology)