Utility of immunohistochemical analysis for cyclo-oxygenase 2 in the differential diagnosis of osteoblastoma and osteosarcoma.

Abstract

AIMS:

To study the immunoexpression of cyclo-oxygenase (COX) 2 in osteoblastomas (OBs) and osteosarcomas (OSs), and to assess the utility of immunohistochemical analysis for COX 2 in the differential diagnosis of the two tumour forms.

METHODS:

The immunohistochemical features of COX 2 were studied in 11 OBs and 30 OSs, including 26 high-grade OSs (16 osteoblastic, 7 chondroblastic, and 3 fibroblastic) and 4 low-grade OSs.

RESULTS:

Tumour cells from all 11 OBs unequivocally showed diffuse, intense and cytoplasmic immunoreactivity for COX 2. Strong cytoplasmic expression of COX 2 was observed in 5 of 26 (19%) high-grade OSs, all chondroblastic. In one osteoblastic-type OS, COX 2 was expressed in the chondroblastic component, but this tumour was considered to be COX 2 negative. No COX 2 expression was noted in atypical osteoblastic cells. Staining in the four low-grade OSs was negative.

CONCLUSION:

The results of immunohistochemical analysis of COX 2 suggest that in addition to the routine histopathological evaluation, COX 2 is a valuable diagnostic marker in the distinction between OB and OS.

• Authors: Ako Hosono, Umio Yamaguchi, Atsushi Makimoto, Makoto Endo, Atsuko Watanabe, Tadakazu Shimoda, Mitsunori Kaya, Tadaki Matsumura, Hiroshi Sonobe, Tomomi Kusumi, Takehiko Yamaguchi, Tadashi Hasegawa
• Journal: Journal of clinical pathology (J Clin Pathol) Vol. 60 Issue 4 Pg. 410-4 (Apr 2007) ISSN: 0021-9746 [Print] England
• PMID: 16822878 (Publication Type: Evaluation Study, Journal Article, Multicenter Study)

Chemical References

• Biomarkers, Tumor
• Cyclooxygenase 2

Topics

• Adolescent
• Adult
• Biomarkers, Tumor (metabolism)
• Bone Neoplasms (diagnosis, pathology)
• Child
• Clinical Enzyme Tests (methods)
• Cyclooxygenase 2 (metabolism)
• Diagnosis, Differential
• Female
• Humans
• Immunoenzyme Techniques
• Male
• Middle Aged
• Osteoblastoma (diagnosis, pathology)
• Osteosarcoma (diagnosis, pathology)