HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Direct DNA sequencing of the rat neu oncogene transmembrane domain reveals no mutation in urinary bladder carcinomas induced by N-butyl-N-(4-hydroxybutyl)nitrosamine, N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide or N-methyl-N-nitrosourea.

Abstract
Epidermal growth factor (EGF) and EGF-related growth factors are present in the urine, and EGF has been identified as a urinary component that enhances urinary bladder tumor formation in rats. Neu oncogene encodes a cell surface receptor similar to the EGF receptor and is known to be activated by a point mutation of DNA that encodes the transmembrane domain of the neu protein (p185). In this study, we examined the possible mutational activation of neu oncogene in 50 urinary bladder transitional cell carcinomas (TCC) induced in F344 rats by the following carcinogenesis models: (i) 0.05% N-butyl-N-(4-hydroxybutyl)nitrosamine (BBN) (4 weeks)----3% uracil (20 weeks); (ii) 0.2% N-[4-(5-nitro-2-furyl)-2-thiazolyl]formamide (FANFT) (6 weeks)----5% sodium saccharin (72 weeks); and (iii) N-methyl-N-nitrosourea (MNU) 20 mg/kg body wt, i.p. twice per week for 4 weeks----3% uracil (20 weeks). The DNA sequence around the transmembrane domain of neu gene was amplified by PCR and sequenced. The results showed no mutation within the examined DNA sequences, indicating that neu oncogene is not activated by a point mutation in the transmembrane domain in urinary bladder carcinomas induced by BBN, FANFT or MNU.
AuthorsT Masui, A M Mann, T L Macatee, E M Garland, T Okamura, R A Smith, S M Cohen
JournalCarcinogenesis (Carcinogenesis) Vol. 12 Issue 10 Pg. 1975-8 (Oct 1991) ISSN: 0143-3334 [Print] UNITED STATES
PMID1682063 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • DNA, Neoplasm
  • Proto-Oncogene Proteins
  • Butylhydroxybutylnitrosamine
  • Uracil
  • Methylnitrosourea
  • FANFT
  • Receptor, ErbB-2
  • Saccharin
Topics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Butylhydroxybutylnitrosamine
  • DNA, Neoplasm (genetics)
  • FANFT
  • Male
  • Membranes (physiology)
  • Methylnitrosourea
  • Molecular Sequence Data
  • Mutation
  • Oncogenes (genetics)
  • Polymerase Chain Reaction
  • Proto-Oncogene Proteins (genetics)
  • Rats
  • Rats, Inbred F344
  • Receptor, ErbB-2
  • Saccharin (adverse effects)
  • Uracil (adverse effects)
  • Urinary Bladder Neoplasms (chemically induced, genetics)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: