Abstract |
Several chemically synthesized compounds were examined for protective effects against the cell damage in tunicamycin-treated human neuroblastoma IMR-32 cells. Among the compounds tested, an antioxidant, Norbergenin-11-caproate (10 microM), exhibited complete protection against the cell growth inhibitory effect of tunicamycin but did not inhibit the induction of Bip/ GRP78 mRNA by tunicamycin. Both norbergenin-11-caproate and alpha-tocopherol completely inhibited the production of reactive oxygen species induced by tunicamycin, however, alpha-tocopherol inhibited tunicamycin-induced cell damage only partially, even at 100 microM. These findings suggest the potential of Norbergenin-11-caproate for therapeutic application in endoplasmic reticulum (ER) stress-dependent diseases implicating a specific mechanism other than anti-oxidative one.
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Authors | Shinya Suzuki, Yui Okuse, Masafumi Kawase, Masufumi Takiguchi, Yoshiyasu Fukuyama, Hironobu Takahashi, Masao Sato |
Journal | Biological & pharmaceutical bulletin
(Biol Pharm Bull)
Vol. 29
Issue 7
Pg. 1335-8
(Jul 2006)
ISSN: 0918-6158 [Print] Japan |
PMID | 16819164
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Benzopyrans
- DNA Primers
- Drugs, Chinese Herbal
- Endoplasmic Reticulum Chaperone BiP
- HSPA5 protein, human
- Heat-Shock Proteins
- Molecular Chaperones
- Reactive Oxygen Species
- Tunicamycin
- norbergenin
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Topics |
- Benzopyrans
(pharmacology)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- DNA Primers
- Drugs, Chinese Herbal
- Endoplasmic Reticulum Chaperone BiP
- Flow Cytometry
- Heat-Shock Proteins
(genetics)
- Humans
- Japan
- Molecular Chaperones
(genetics)
- Neuroblastoma
- Reactive Oxygen Species
(metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
- Tunicamycin
(toxicity)
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