Abstract |
A new fluorescent thiol reagent, dansyl-aminophenylmercuric acetate ( DAPMA), was applied to the diagnosis of homocystinuria, a disorder which can be associated with vascular disease at an early age. DAPMA was added to urine containing metabisulphite and the resulting fluorescent derivatives were extracted on a cyclohexyl silica column and separated by thin-layer chromatography. 102 coded samples were tested. The derivative of homocysteine was easily identified in samples from 4 children with homocystinuria but was absent from all samples from normal subjects and patients with unrelated disorders. Other thiols ( cysteine, acetylcysteine, mercaptolactate, thiosulphate, and thiocyanate) were also identified in urine from healthy fasting subjects.
|
Authors | J L Maddocks, J MacLachlan |
Journal | Lancet (London, England)
(Lancet)
Vol. 338
Issue 8774
Pg. 1043-4
(Oct 26 1991)
ISSN: 0140-6736 [Print] England |
PMID | 1681358
(Publication Type: Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Sulfhydryl Compounds
- 4-dansyl aminophenylmercuric acetate
- Phenylmercuric Acetate
|
Topics |
- Child
- Chromatography, Thin Layer
(methods)
- Fluorescence
- Homocystinuria
(diagnosis)
- Humans
- Phenylmercuric Acetate
(analogs & derivatives, chemistry)
- Sulfhydryl Compounds
(urine)
|