Abstract |
An experimental study was designed to investigate the efficacy of BMAP-27, a compound of the cathelicidin family, in neutralizing Escherichia coli 0111:B4 lipopolysaccharide (LPS) in bile duct-ligated mice. Main outcome measures were: endotoxin and TNF-alpha concentrations in plasma, evidence of bacterial translocation in blood and peritoneum, and lethality. Adult male BALB/c mice were injected intraperitoneally with 2 mg/kg E. coli 0111:B4 LPS 1 week after sham operation or bile duct ligation (BDL). Six groups were studied: sham with placebo, sham with 120 mg/kg tazobactam-piperacillin (TZP), sham with 1 mg/kg BMAP-27, BDL with placebo, BDL with 120 mg/kg TZP, and BDL with 1mg/kg BMAP-27. After LPS, TNF-alpha plasma levels were significantly higher in BDL mice compared to sham-operated animals. BMAP-27 achieved a significant reduction of plasma endotoxin and TNF-alpha concentration when compared with placebo- and TZP-treated groups. On the other hand, both TZP and BMAP-27 significantly reduced the bacterial growth compared with saline treatment. Finally, LPS induced 60% and 55% lethality in BDL placebo- and TZP-treated treated mice and no lethality in sham-operated mice, while only BMAP-27 significantly reduced the lethality to 10%. In light of its dual antimicrobial and anti- endotoxin properties, BMAP-27 could be an interesting compound to inhibit bacterial translocation and endotoxin release in obstructive jaundice.
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Authors | Roberto Ghiselli, Oscar Cirioni, Andrea Giacometti, Federico Mocchegiani, Fiorenza Orlando, Cristina Bergnach, Barbara Skerlavaj, Carmela Silvestri, Agnese Della Vittoria, Margherita Zanetti, Marco Rocchi, Giorgio Scalise, Vittorio Saba |
Journal | Peptides
(Peptides)
Vol. 27
Issue 11
Pg. 2592-9
(Nov 2006)
ISSN: 0196-9781 [Print] United States |
PMID | 16806583
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antimicrobial Cationic Peptides
- BMAP-27
- Lipopolysaccharides
- Proteins
- Tumor Necrosis Factor-alpha
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Topics |
- Animals
- Antimicrobial Cationic Peptides
(therapeutic use)
- Bacterial Translocation
(drug effects)
- Disease Models, Animal
- Jaundice, Obstructive
(chemically induced, drug therapy)
- Lipopolysaccharides
- Male
- Mice
- Mice, Inbred BALB C
- Proteins
(metabolism, therapeutic use)
- Tumor Necrosis Factor-alpha
(blood)
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