Effects of atorvastatin and apoA-I/phosphatidylcholine discs on triglyceride-rich lipoprotein subfractions as characterized by capillary isotachophoresis.

Abstract

BACKGROUND:

The present study examined the effects of atorvastatin and the in vitro effect of apolipoprotein (apo) A-I/phosphatidylcholine (POPC) discs on charge-based triglyceride-rich lipoprotein (TRL) subfractions in a patient with type III hyperlipoproteinemia (HLP) and the apoE2/2 phenotype.

METHODS:

Charge-based lipoprotein subfractions were characterized by capillary isotachophoresis (cITP). cITP analysis was performed using plasma that had been prestained with a lipophilic dye on a Beckman P/ACE MDQ system.

RESULTS:

Treatment with atorvastatin for 4 weeks markedly decreased the slow (s)-migrating TRL subfraction and both fast- and slow-migrating low-density lipoprotein (LDL) subfractions, but did not affect the fast (f)-migrating TRL subfraction in this patient. ApoA-I/POPC discs consisted of two major charge-based subfractions that had the mobility of cITP fTRL and sTRL. Incubation of plasma from this patient in the presence of apoA-I/POPC discs caused not only a reduction in cITP fast- and intermediate-migrating HDL and an increase in cITP sHDL but also a reduction in fTRL and sTRL and an increase in sLDL.

CONCLUSION:

Atorvastatin and apoA-I/POPC discs decreased cITP TRL subfractions in a complementary manner, suggesting that the combination of apoA-I/POPC discs and atorvastatin could be a promising therapeutic approach for hypertriglyceridemia.

• Authors: Bo Zhang, Ritsuko Katafuchi, Hiroaki Arishima, Akira Matsunaga, Kerry-Anne Rye, Keijiro Saku
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 373 Issue 1-2 Pg. 55-61 (Nov 2006) ISSN: 0009-8981 [Print] Netherlands
• PMID: 16806136 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Apolipoprotein A-I
• Apolipoprotein E2
• Heptanoic Acids
• Lipoproteins
• Macromolecular Substances
• Phosphatidylcholines
• Pyrroles
• Triglycerides
• Atorvastatin

Topics

• Adult
• Apolipoprotein A-I (chemistry, pharmacology)
• Apolipoprotein E2 (genetics)
• Atorvastatin
• Electrophoresis, Capillary (methods)
• Heptanoic Acids (administration & dosage, pharmacology)
• Humans
• Hyperlipoproteinemia Type III (blood, drug therapy, genetics)
• Lipoproteins (blood, chemistry, drug effects)
• Macromolecular Substances (chemistry, pharmacology)
• Male
• Phenotype
• Phosphatidylcholines (chemistry, pharmacology)
• Pyrroles (administration & dosage, pharmacology)
• Sensitivity and Specificity
• Time Factors
• Triglycerides (analysis)