Human laminin-5 and laminin-10 mediated gene expression of prostate carcinoma cells.

Abstract

In prostate cancer progression, the basal lamina switches from predominantly laminin-5 to laminin-10. DU-145 prostate cancer cells were treated with either soluble laminin-5 (20 ng/ml) or laminin-10 (1 microg/ml) for 6, 24, and 48 hr. Total RNA was harvested for a 7,500 human cDNA microarray. Hybridizations were carried out in accordance with a 10 sample analysis of variance (ANOVA) statistical model. One thousand one hundred sixteen genes had measurable expression 2 standard deviations above background and 50% of spots for any given sample for all hybridizations were positive. Expression values of significantly varying genes were clustered and a list of 408 genes (P < 0.05) with a 1.5 or greater fold change in at least one time point were chosen for further analysis. Seventy eight changed in a time-dependent manner with laminin-10 treatment, 85 changed with laminin-5, and 13 showed changes with both treatments. The 408 genes that passed a paired t-test in at least one time-dependent category were further analyzed using Pathway Miner. One of the largest gene association networks involved signal transduction in the growth factor-MAP kinase pathways. EGFR was validated by real-time PCR and laminin-10 mediated cell adhesion activated EGFR in DU-145 cells. Both laminins appear to be important signal transducers in prostate cancer.

• Authors: Robert Calaluce, Shaleen K Beck, Elisabeth L Bair, Ritu Pandey, Kevin A Greer, Adam M Hoying, James B Hoying, David W Mount, Raymond B Nagle
• Journal: The Prostate (Prostate) Vol. 66 Issue 13 Pg. 1381-90 (Sep 15 2006) ISSN: 0270-4137 [Print] United States
• PMID: 16804886 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)

Chemical References

• Cell Adhesion Molecules
• DNA, Neoplasm
• Laminin
• RNA, Neoplasm
• kalinin
• laminin 10
• ErbB Receptors
• Calpain

Topics

• Calpain (genetics, metabolism)
• Cell Adhesion Molecules (physiology)
• Cell Line, Tumor
• DNA, Neoplasm (genetics)
• ErbB Receptors (genetics, metabolism)
• Gene Expression Profiling
• Gene Expression Regulation, Neoplastic (drug effects, genetics)
• Humans
• Laminin (physiology)
• MAP Kinase Signaling System (genetics)
• Male
• Oligonucleotide Array Sequence Analysis
• Prostatic Neoplasms (genetics, metabolism, pathology)
• RNA, Neoplasm (genetics)
• Signal Transduction (genetics)
• Time Factors