Abstract | BACKGROUND: METHODS: Male Sprague-Dawley rats (n = 45, 350 +/- 30 g) were randomly assigned to 5 groups of 9 animals each. After anesthesia with isoflurane, the femoral artery and vein were surgically cannulated. Hemorrhagic shock was induced by withdrawing blood through the arterial cannula until the mean arterial pressure (MAP) was 25-30 mm Hg and maintained at the level for 30 minutes with further withdrawals. Resuscitation was carried out by giving 21 mL/kg Ringer's lactate (LR) and returning the shed blood, with or without the initial administration of crocetin (2 mg/kg). Controls were normal ( anesthesia only), sham (surgical preparation), and shock (preparation and shock). Rats were killed 30 minutes after completion of resuscitation. Liver samples were collected for reverse transcription-polymerase chain reaction (RT-PCR) analysis of mRNA ( TNF-alpha, IL-1beta, iNOS, and beta-actin). RESULTS: CONCLUSIONS:
|
Authors | Rongjie Yang, Xiaoyu Tan, Ann M Thomas, Jing Shen, Nilofer Qureshi, David C Morrison, Charles W Van Way 3rd |
Journal | JPEN. Journal of parenteral and enteral nutrition
(JPEN J Parenter Enteral Nutr)
2006 Jul-Aug
Vol. 30
Issue 4
Pg. 297-301
ISSN: 0148-6071 [Print] United States |
PMID | 16804126
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
|
Chemical References |
- Antioxidants
- Interleukin-1beta
- RNA, Messenger
- Tumor Necrosis Factor-alpha
- trans-sodium crocetinate
- Vitamin A
- Carotenoids
- Nitric Oxide Synthase Type II
|
Topics |
- Animals
- Antioxidants
(therapeutic use)
- Carotenoids
(therapeutic use)
- Interleukin-1beta
(metabolism)
- Liver
(enzymology, metabolism)
- Male
- Nitric Oxide Synthase Type II
(metabolism)
- RNA, Messenger
(analysis, antagonists & inhibitors, metabolism)
- Random Allocation
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Shock, Hemorrhagic
(drug therapy)
- Tumor Necrosis Factor-alpha
(metabolism)
- Vitamin A
(analogs & derivatives)
|