The complete genome sequence of a chronic atrophic gastritis Helicobacter pylori strain: evolution during disease progression.

Abstract

Helicobacter pylori produces acute superficial gastritis in nearly all of its human hosts. However, a subset of individuals develops chronic atrophic gastritis (ChAG), a condition characterized in part by diminished numbers of acid-producing parietal cells and increased risk for development of gastric adenocarcinoma. Previously, we used a gnotobiotic transgenic mouse model with an engineered ablation of parietal cells to show that loss of parietal cells provides an opportunity for a H. pylori isolate from a patient with ChAG (HPAG1) to bind to, enter, and persist within gastric stem cells. This finding raises the question of how ChAG influences H. pylori genome evolution, physiology, and tumorigenesis. Here we describe the 1,596,366-bp HPAG1 genome. Custom HPAG1 Affymetrix GeneChips, representing 99.6% of its predicted ORFs, were used for whole-genome genotyping of additional H. pylori ChAG isolates obtained from Swedish patients enrolled in a case-control study of gastric cancer, as well as ChAG- and cancer-associated isolates from an individual who progressed from ChAG to gastric adenocarcinoma. The results reveal a shared gene signature among ChAG strains, as well as genes that may have been lost or gained during progression to adenocarcinoma. Whole-genome transcriptional profiling of HPAG1's response to acid during in vitro growth indicates that genes encoding components of metal uptake and utilization pathways, outer membrane proteins, and virulence factors are among those associated with H. pylori's adaptation to ChAG.

• Authors: Jung D Oh, Helene Kling-Bäckhed, Marios Giannakis, Jian Xu, Robert S Fulton, Lucinda A Fulton, Holland S Cordum, Chunyan Wang, Glendoria Elliott, Jennifer Edwards, Elaine R Mardis, Lars G Engstrand, Jeffrey I Gordon
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 103 Issue 26 Pg. 9999-10004 (Jun 27 2006) ISSN: 0027-8424 [Print] United States
• PMID: 16788065 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Topics

• Adenocarcinoma (microbiology)
• Base Pairing
• Chronic Disease
• Disease Progression
• Gastritis, Atrophic (microbiology)
• Gene Expression Profiling
• Gene Expression Regulation, Bacterial
• Genome, Bacterial (genetics)
• Genomic Instability
• Genotype
• Helicobacter pylori (drug effects, genetics, growth & development)
• Humans
• Hydrogen-Ion Concentration
• Molecular Sequence Data
• Oligonucleotide Array Sequence Analysis
• Sequence Analysis, DNA
• Stomach Neoplasms (microbiology)