Biological and environmental factors affecting ultrasound-induced hemolysis in vitro: 5. Temperature.

Abstract

This research project tested the hypothesis that cold-equilibrated (approximately 0 degrees C) human erythrocytes in vitro in the presence of an ultrasound contrast agent (Albunex) will undergo greater ultrasound-induced hemolysis than physiologically equilibrated (37 degrees C) human erythrocytes in vitro because of a temperature-related transition in membrane fluidity leading to increased fragility. First, it was shown that cold-equilibrated erythrocytes are more susceptible to mechanically induced hemolysis than physiologically equilibrated erythrocytes. Second, when adjustments were made for (1) temperature-dependent efficiencies of a 1-MHz transducer (200 micros pulse length, 20 ms interpulse interval, 30 s exposure duration) such that when cold or physiological temperatures were employed, there were equivalent acoustic outputs in terms of peak negative pressure (MPa P-) and (2) comparable viscosities of the 0 and 37 degrees C blood plasmas, the cold (approximately 0 degrees C) erythrocytes displayed substantially greater amounts of ultrasound-induced hemolysis than the physiological (37 degrees C) erythrocytes. The data supported the hypothesis.

• Authors: Morton W Miller, Charles C Church, Cecille Labuda, Salvatore Mazza, Jason Raymond
• Journal: Ultrasound in medicine & biology (Ultrasound Med Biol) Vol. 32 Issue 6 Pg. 893-904 (Jun 2006) ISSN: 0301-5629 [Print] England
• PMID: 16785011 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Oxygen

Topics

• Adult
• Blood Viscosity
• Erythrocyte Membrane (physiology)
• Hemolysis
• Humans
• In Vitro Techniques
• Oxygen (blood)
• Partial Pressure
• Temperature
• Transducers
• Ultrasonography