Novel ELISA system for detection of N-ERC/mesothelin in the sera of mesothelioma patients.

Abstract

We have developed a novel enzyme-linked immunosorbent assay (ELISA) system for the detection of N-ERC/mesothelin in the serum of mesothelioma patients and have begun to examine its clinical usefulness. N-ERC/mesothelin is a 31-kDa protein that forms the N-terminal fragment of the full-length 71-kDa ERC/mesothelin protein, and is physiologically secreted into the blood of mesothelioma patients where it can be detected using our sandwich ELISA containing two antibodies (rabbit polyclonal anti-ERC/mesothelin antibody-282 and mouse monoclonal antibody 7E7). Our ELISA system has thus far detected much higher serum levels of N-ERC/mesothelin in mesothelioma patients than in healthy controls or patients with other lung or pleural diseases. In conclusion, N-ERC/mesothelin is a promising candidate tumor marker for mesothelioma.

• Authors: Kazu Shiomi, Hideaki Miyamoto, Tatsuya Segawa, Yoshiaki Hagiwara, Akinobu Ota, Masahiro Maeda, Kazuhisa Takahashi, Kimihiko Masuda, Yukinori Sakao, Okio Hino
• Journal: Cancer science (Cancer Sci) Vol. 97 Issue 9 Pg. 928-32 (Sep 2006) ISSN: 1347-9032 [Print] England
• PMID: 16776777 (Publication Type: Journal Article)

Chemical References

• Antibodies
• Antibodies, Monoclonal
• Biomarkers, Tumor
• GPI-Linked Proteins
• Membrane Glycoproteins
• Msln protein, mouse
• Mesothelin

Topics

• Animals
• Antibodies
• Antibodies, Monoclonal
• Biomarkers, Tumor (blood)
• Blotting, Western
• COS Cells
• Chlorocebus aethiops
• Enzyme-Linked Immunosorbent Assay (methods)
• GPI-Linked Proteins
• HeLa Cells
• Humans
• Immunohistochemistry
• Membrane Glycoproteins (blood)
• Mesothelin
• Mesothelioma (blood, diagnosis)
• Mice
• Rabbits