Abstract |
Essentially all eukaryotic cells, including murine lymphomas, express surface proteins, such as Thy-1, which are anchored by a phosphoinositol mannolipid. Putative mannolipid anchor precursors can be detected in these cells. Six distinct Thy-1-negative lymphoma mutants lack complete mannolipids, and three mutants synthesize atypical mannolipids. The absence of complete mannolipids can account for the lack of expression of multiple mannolipid-anchored proteins and may also account for the lack of lipid anchoring in the human disease paroxysmal nocturnal hemoglobinuria. Structural information on the mannolipids of wild-type and mutant cells indicates that anchor biosynthesis in these cells may involve both transmembrane flip-flop of intermediates and a deacylation step.
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Authors | P Lemansky, D K Gupta, S Meyale, G Tucker, A M Tartakoff |
Journal | Molecular and cellular biology
(Mol Cell Biol)
Vol. 11
Issue 8
Pg. 3879-85
(Aug 1991)
ISSN: 0270-7306 [Print] United States |
PMID | 1677158
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, Surface
- Glycolipids
- Glycosylphosphatidylinositols
- Phosphatidylinositols
- Thy-1 Antigens
- Mannose
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Topics |
- Animals
- Antigens, Surface
(genetics)
- Carbohydrate Sequence
- Cell Line
- Glycolipids
(biosynthesis, isolation & purification, metabolism)
- Glycosylphosphatidylinositols
- Lymphoma
(genetics, immunology)
- Mannose
(isolation & purification, metabolism)
- Mice
- Molecular Sequence Data
- Mutation
- Phenotype
- Phosphatidylinositols
(metabolism)
- Thy-1 Antigens
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