Human lung adenocarcinoma cell lines with different lung colonization potential (LCP), and a correlation between expression of sialosyl dimeric Le(x) (defined by MAb FH6) and LCP.

Abstract

Human lung adenocarcinoma sub-cell lines HAL-8, HAL-24 and HAL-33, showing different lung colonization potential (LCP), were established from human lung adenocarcinoma cell line KUM-LK-2 using repeated cloning with limiting dilution technique. Cell lines HAL-8 and -33 were characterized by high and low LCP, respectively, while HAL-24 did not give rise to lung colonies. The cell surface protein and carbohydrate profiles were determined by cell surface labeling (with lactoperoxidase-dependent 125I-iodination and galactose oxidase-NaB3H4, respectively) followed by SDS-gel electrophoresis. Various carbohydrate epitopes expressed at the cell surface were analysed by cytofluorometry using various monoclonal antibodies (MAbs) directed to Le(x), sialosyl-Le(x), sialosyl dimeric Le(x), T, Tn and sialosyl-Tn structures, which are often reported as being highly expressed in a variety of human cancers, particularly adenocarcinoma. Expression of sialosyl dimeric Le(x) (defined by MAb FH6) was high on HAL-8, moderate on HAL-33, and relatively low on HAL-24. In contrast, each of the three lines showed essentially equal expression (as determined by MAb reactivity) of sialosyl-Tn (defined by MAb TKH2), Le(x) (defined by MAb SH1), and Tn (defined by MAb 1E3). The cell lines showed extremely weak expression of T (defined by MAb HH8). LCP of HAL-8 and -33 was completely inhibited by sialidase treatment of cells. It is suggested that higher expression of sialosyl dimeric Le(x) (defined by MAb FH6) in HAL-8 cells may play an important role in higher potential of blood-borne lung colonization.

• Authors: H Inufusa, N Kojima, M Yasutomi, S Hakomori
• Journal: Clinical & experimental metastasis (Clin Exp Metastasis) 1991 May-Jun Vol. 9 Issue 3 Pg. 245-57 ISSN: 0262-0898 [Print] Netherlands
• PMID: 1676353 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Antibodies, Monoclonal
• Antigens, Surface
• Antigens, Tumor-Associated, Carbohydrate
• Lewis X Antigen
• sialosyl dimeric Le(x) antigen
• Neuraminidase

Topics

• Adenocarcinoma (metabolism, pathology)
• Animals
• Antibodies, Monoclonal (metabolism)
• Antigens, Surface (metabolism)
• Antigens, Tumor-Associated, Carbohydrate (metabolism)
• Carbohydrate Metabolism
• Carbohydrate Sequence
• Flow Cytometry
• Fluorescent Antibody Technique
• Glycosylation
• Humans
• Lewis X Antigen (metabolism)
• Lung Neoplasms (metabolism, pathology)
• Mice
• Mice, Nude
• Molecular Sequence Data
• Neoplasm Metastasis (pathology)
• Neuraminidase (antagonists & inhibitors, pharmacology)
• Tumor Cells, Cultured