[Induction of apoptosis of tumor multidrug resistance cell by uvarigrin and its mechanism].

Abstract

AIM:

To study the effect of uvarigrin on mitochondrial dependent pathway during the apoptosis induced by it in MDR KBv200 cells and their parental sensitive KB cells.

METHODS:

MTT assay was used to detect the cytotoxic effect of uvarigrin on KBv200 and KB cells. Annexin V FITC staining identified uvarigrin-induced apoptosis in KBv200 and KB cells. These cells underwent incubation with DCFH-DA, or DiOC6, followed by flowcytometry for the measurement of reactive oxygen species (ROS) and mitochondrial membrane potential (deltapsim), respectively. The Western blotting analysis was performed on Caspase-9 activation.

RESULTS:

Uvarigrin inhibited the growth of KBv200 cells and KB cells in vitro. Most of the uvarigrin-induced cells death was found to be due to apoptosis, as determined by Annexin V FITC staining. During the apoptosis, the level of ROS increased while the level of deltapsim decreased in a time-dependent manner. Uvarigrin triggered Caspase-9 activation.

CONCLUSION:

Uvarigrin induced apoptosis in KBv200 cells and KB cells probably through a mitochondria-dependent pathway.

• Authors: Yan-fang Li, Yong-ju Liang, Zhi Shi, Li-ming Chen, Yan Ding, Li-wu Fu
• Journal: Yao xue xue bao = Acta pharmaceutica Sinica (Yao Xue Xue Bao) Vol. 41 Issue 3 Pg. 252-6 (Mar 2006) ISSN: 0513-4870 [Print] China
• PMID: 16758998 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Furans
• Lactones
• Reactive Oxygen Species
• uvarigrin
• Caspase 9

Topics

• Antineoplastic Agents, Phytogenic (administration & dosage, isolation & purification, pharmacology)
• Apoptosis (drug effects)
• Caspase 9 (metabolism)
• Cell Proliferation (drug effects)
• Dose-Response Relationship, Drug
• Drug Resistance, Multiple
• Drug Resistance, Neoplasm
• Furans (administration & dosage, isolation & purification, pharmacology)
• Humans
• KB Cells
• Lactones (administration & dosage, isolation & purification, pharmacology)
• Membrane Potentials (drug effects)
• Mitochondria (physiology)
• Plants, Medicinal (chemistry)
• Reactive Oxygen Species (metabolism)
• Uvaria (chemistry)