Effects of Gekko sulfated polysaccharide on the proliferation and differentiation of hepatic cancer cell line.

Abstract

Gekko swinhonis Gūenther has been used as an anti-cancer drug in traditional Chinese medicine. Gekko sulfated polysaccharide (Gepsin) was investigated for its activity in hepatocellular carcinoma. Hepatocarcinoma cell line (Bel-7402) and liver cell line (L-02) were exposed to Gepsin (100 microg/ml and 10 microg/ml). Gepsin did not suppress the proliferation and viability of normal liver L-02 cells, but strongly inhibited the proliferation of hepatocarcinoma Bel-7402 cells. Gepsin did not induce the apoptosis of Bel-7402 cells, but blocked cells in G2/M phase. Treated with Gepsin, Bel-7402 cells showed ultrastructural features of differentiation. AFP secretion decreased while ALB secretion increased markedly on Gepsin-treated cells. The data show that Gepsin suppressed the proliferation and induced differentiation of hepatocarcinoma, but the toxicity to normal liver cells was negligible.

• Authors: XiongZhi Wu, Dan Chen, Guang-Ru Xie
• Journal: Cell biology international (Cell Biol Int) Vol. 30 Issue 8 Pg. 659-64 (Aug 2006) ISSN: 1065-6995 [Print] England
• PMID: 16757189 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Polysaccharides
• Sulfates
• Tissue Extracts
• gepsin

Topics

• Animals
• Antineoplastic Agents (chemistry, metabolism, pharmacology)
• Apoptosis (drug effects)
• Carcinoma, Hepatocellular
• Cell Cycle (drug effects)
• Cell Differentiation (drug effects)
• Cell Line, Tumor
• Cell Proliferation (drug effects)
• Cell Shape
• Cell Survival (drug effects)
• Humans
• Liver Neoplasms
• Lizards
• Medicine, Chinese Traditional
• Polysaccharides (chemistry, metabolism, pharmacology)
• Sulfates (chemistry)
• Tissue Extracts (chemistry, pharmacology)