Abstract |
In the present study, the expression of cyclin E and kinase p34 cdc2 was investigated in preinvasive bladder tumors. The study material consisted of bladder sections (grades: GI--16 cases, GII--10, and GIII--12) collected from 38 patients in the course of the tumor electroresection. Immunohistochemical examinations were carried out with immunoperoxidase method. Antigens were labeled with NCL- CYCLIN E or NCL-p34 cdc2 monoclonal antibodies (Novocastra, UK). Positive reaction was demonstrated using ABC-universal Kit (Novocastra, UK). Differences in the protein expression in relation to the tumor grade were determined with a non-parametric Mann-Whitney's test. Increasing grade of tumors was associated with down regulation of cyclin E visible as lower percentage of cyclin E-positive cells. These changes were statistically significant for GI group as compared to groups GII and GIII (p<0.001). There were no differences between the study groups in the p34 protein expression. Cyclin E expression was inversely correlated with tumor grade therefore may be helpful in establishing therapeutic procedure.
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Authors | Piotr Kaczmarek, Dariusz Tosik, Ewa Majewska, Zbigniew Baj |
Journal | Polish journal of pathology : official journal of the Polish Society of Pathologists
(Pol J Pathol)
Vol. 57
Issue 1
Pg. 41-4
( 2006)
ISSN: 1233-9687 [Print] Poland |
PMID | 16739882
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Cyclin E
- CDC2 Protein Kinase
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Topics |
- Aged
- Biomarkers, Tumor
(metabolism)
- CDC2 Protein Kinase
(metabolism)
- Carcinoma, Transitional Cell
(metabolism, pathology, surgery)
- Cyclin E
(metabolism)
- Down-Regulation
- Female
- Humans
- Male
- Middle Aged
- Neoplasm Staging
- Urinary Bladder Neoplasms
(metabolism, pathology, surgery)
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