[Effects of acitretin on the expression of signaling pathway-related genes in epidermal squamous-cell carcinoma cells].

Abstract

OBJECTIVE:

To study the effects of acitretin on the expression of signaling pathway-related genes in an epidermal squamous-cell carcinoma cell line.

METHODS:

The mRNA expression levels of STAT3, cyclin D1 and p42/44MAPK were detected in a human epidermal carcinoma cell line A431 by RT-PCR. Their expressions at protein level were studied by Western blot. The expression levels were studied in cells treated with or without 10(-5) mol/L acitretin at different time intervals.

RESULTS:

(1) Acitretin could significantly inhibit the expression of STAT3 and cyclin D1 mRNA in a time-dependent manner (P < 0.05). The STAT3 and cyclin D1 protein expression levels were down-regulated. Acitretin could also down-regulate the p42/4MAPK mRNA expression. (2) After incubation with acitretin, the mRNA level of cyclin D1 cells was positively correlated with that of STAT3 (P < 0.05). The cyclin D1 protein level was also positively correlated with that of STAT3 (P < 0.05). However there was no correlation of mRNA levels between cyclin D1 and p42/44MAPK.

CONCLUSION:

Regulation of the Jak/STAT3 signaling pathway may play an important role in the effect of acitretin on epidermal squamous-cell carcinoma cells. The abnormal expression of STAT3 can be regarded as a prerequisite for acitretin treatment effect.

• Authors: Sui-qing Cai, Li-rong Chen, Min Zheng
• Journal: Zhonghua zhong liu za zhi [Chinese journal of oncology] (Zhonghua Zhong Liu Za Zhi) Vol. 28 Issue 1 Pg. 21-4 (Jan 2006) ISSN: 0253-3766 [Print] China
• PMID: 16737614 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• RNA, Messenger
• STAT3 Transcription Factor
• STAT3 protein, human
• Cyclin D1
• Mitogen-Activated Protein Kinase 1
• Mitogen-Activated Protein Kinase 3
• Acitretin

Topics

• Acitretin (pharmacology)
• Antineoplastic Agents (pharmacology)
• Carcinoma, Squamous Cell (metabolism, pathology)
• Cell Line, Tumor
• Cyclin D1 (biosynthesis, genetics)
• Down-Regulation
• Gene Expression Regulation, Neoplastic
• Humans
• Mitogen-Activated Protein Kinase 1 (biosynthesis, genetics)
• Mitogen-Activated Protein Kinase 3 (biosynthesis, genetics)
• RNA, Messenger (biosynthesis, genetics)
• STAT3 Transcription Factor (biosynthesis, genetics)
• Signal Transduction