Abstract |
Human immune responses to modern synthetic and recombinant peptide vaccines administered with the standard adjuvant, aluminum hydroxide, tend to be poor, hence the search for better adjuvants. Antibody responses to a Plasmodium falciparum circumsporozoite (CS) protein vaccine, R32NS1(81), administered with an adjuvant containing cell-wall skeleton of mycobacteria and monophosphoryl lipid A in squalane (MPL/CWS) have been compared to responses to the same immunogen administered with aluminum hydroxide. 2 weeks after the third dose the following indices were greater in the 5 patients who received MPL/CWS than in controls (p less than 0.05): the geometric mean concentration (2.0 vs 25.4 microgram/ml) and avidity index of antibodies to the P falciparum CS protein by ELISA, the geometric mean titre to P falciparum sporozoites by IFAT (1/115 vs 1/1600), and the geometric mean inhibition of sporozoite invasion of hepatoma cells in vitro (37.6 vs 90.3%). For R32NS1(81) MPL/CWS is superior to aluminum hydroxide as an adjuvant, and the data support the evaluation of this complex as an adjuvant for other vaccines.
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Authors | L S Rickman, D M Gordon, R Wistar Jr, U Krzych, M Gross, M R Hollingdale, J E Egan, J D Chulay, S L Hoffman |
Journal | Lancet (London, England)
(Lancet)
Vol. 337
Issue 8748
Pg. 998-1001
(Apr 27 1991)
ISSN: 0140-6736 [Print] England |
PMID | 1673211
(Publication Type: Clinical Trial, Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Adjuvants, Immunologic
- Antibodies, Protozoan
- Immunoglobulin G
- Lipid A
- Malaria Vaccines
- Protozoan Vaccines
- R 32NS1(81)
- Recombinant Fusion Proteins
- Vaccines, Synthetic
- Squalene
- monophosphoryl lipid A
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Topics |
- Adjuvants, Immunologic
(administration & dosage)
- Adult
- Animals
- Antibodies, Protozoan
(analysis)
- Antibody Affinity
(drug effects)
- Cell Wall
(microbiology)
- Humans
- Immunoglobulin G
(analysis)
- Lipid A
(administration & dosage, analogs & derivatives)
- Malaria Vaccines
- Male
- Mycobacterium
(cytology)
- Plasmodium falciparum
(immunology)
- Protozoan Vaccines
(administration & dosage, adverse effects, immunology)
- Recombinant Fusion Proteins
- Squalene
(administration & dosage)
- Vaccines, Synthetic
(adverse effects, immunology)
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