Reactivity of anti-CD15 monoclonal antibody PM-81 with breast cancer and elimination of breast cancer cells from human bone marrow by PM-81 and immunomagnetic beads.

Abstract

The CD15 carbohydrate antigen, lacto-N-fucopentaose III is expressed on a variety of human cancer cells including acute myeloid leukemia, small cell carcinoma of the lung, and colorectal carcinomas. We have found that cells from breast cancer cell lines and patient-derived tissue are strongly CD15 positive, as seen by binding to the PM-81 monoclonal antibody. In this report we show that monoclonal antibody PM-81 and immunomagnetic beads can remove breast cancer cells from bone marrow and thus be used as "purging" agents for autologous bone marrow transplantation. PM-81 and immunomagnetic beads removed up to 3 log of SK-BR-3 and MCF7 breast carcinoma cell line cells while minimally affecting normal hematopoietic progenitor cells. This technique may be useful for purging marrow for autologous bone marrow transplantation in breast cancer.

• Authors: J J Vredenburgh, W Simpson, V A Memoli, E D Ball
• Journal: Cancer research (Cancer Res) Vol. 51 Issue 9 Pg. 2451-5 (May 01 1991) ISSN: 0008-5472 [Print] United States
• PMID: 1673088 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Monoclonal
• Antigens, Differentiation
• Antigens, Neoplasm
• Lewis X Antigen

Topics

• Antibodies, Monoclonal (immunology)
• Antigens, Differentiation (immunology)
• Antigens, Neoplasm (immunology)
• Bone Marrow Cells
• Bone Marrow Transplantation
• Breast Neoplasms (immunology, pathology, therapy)
• Cell Separation (methods)
• Humans
• Lewis X Antigen
• Magnetics