Abstract | OBJECTIVE: METHODS: In vitro-transcribed U1 snRNA or hY1RNA and lipofectin were added to peripheral blood mononuclear cell (PBMC) cultures. Purified U1 snRNP particles and IgG from SLE patients (SLE- IgG) were added to cultures of PBMCs, enriched monocytes, or natural interferon-producing cells (NIPCs); the latter are also known as plasmacytoid dendritic cells (pDC). Cells were double-stained for IFNalpha and either blood dendritic cell antigen 2 (NIPCs/pDC) or CD14 (monocytes) and then analyzed by flow cytometry. In some experiments, RNase or inhibitors of Fc gamma receptor IIa (Fc gammaRIIa) (specific antibodies), endocytosis ( chloroquine, bafilomycin A), or Toll-like receptors (TLRs; oligodeoxynucleotide 2088) were used. The produced IFNalpha was measured by immunoassay. RESULTS: Lipofected U1 snRNA and hY1RNA both induced IFNalpha production in monocytes, but not in NIPC/pDC. In contrast, U1 snRNP combined with SLE- IgG induced IFNalpha production only in NIPCs/pDC, and this response was decreased by RNase treatment or inhibition of the Fc gammaRIIa, the endocytosis pathways, or the TLRs. CONCLUSION:
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Authors | Tanja Lövgren, Maija-Leena Eloranta, Berthold Kastner, Marie Wahren-Herlenius, Gunnar V Alm, Lars Rönnblom |
Journal | Arthritis and rheumatism
(Arthritis Rheum)
Vol. 54
Issue 6
Pg. 1917-27
(Jun 2006)
ISSN: 0004-3591 [Print] United States |
PMID | 16729300
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigen-Antibody Complex
- Antigens, CD
- Autoantigens
- Fc gamma receptor IIA
- Immunoglobulin G
- Interferon-alpha
- Phosphatidylethanolamines
- RNA, Small Nuclear
- Receptors, IgG
- U1 small nuclear RNA
- 1,2-dielaidoylphosphatidylethanolamine
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Topics |
- Antigen-Antibody Complex
(immunology)
- Antigens, CD
(immunology)
- Autoantigens
(immunology)
- Cells, Cultured
- Flow Cytometry
- Humans
- Immunoglobulin G
(immunology)
- Interferon-alpha
(biosynthesis)
- Lupus Erythematosus, Systemic
(immunology)
- Monocytes
(immunology)
- Phosphatidylethanolamines
- RNA, Small Nuclear
(immunology)
- Receptors, IgG
(antagonists & inhibitors, immunology)
- Sjogren's Syndrome
(immunology)
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