Abstract |
The correlation of c-Myc expression with resveratrol-induced turnover of medulloblastoma cells was investigated in this study by checking (1) c-Myc expression in medulloblastoma tissues and cell lines (UW228-2 and UW228-3), (2) the in vitro effect of resveratrol on c-Myc expression and (3) the influences of c-Myc inhibition in cell growth and survival. Immunohistochemical staining of human medulloblastomas and noncancerous cerebellar tissues revealed that 8 out of 11 tumor tissues (72.7%) expressed c-Myc, in which 4 cases (50%) showed intensified nuclear labeling. RT-PCR, Western blotting, immunocytochemical and immunofluorescence stainings revealed c-Myc downregulation accompanied with growth suppression and apoptosis. Flow cytometry analysis showed S phase arrest in resveratrol-treated cell populations. Transfection of c-Myc directed antisense oligonucleotides to the cultured medulloblastoma cells could reduce c-Myc expression, inhibit cell growth and arrest the cell cycle at S phase. Our results thus for the first time demonstrate that c-Myc downregulation is a critical molecular event of resveratrol-mediated anti- medulloblastoma activity, which is closely associated with growth suppression, cell cycle arrest and apoptosis of medulloblastoma cells.
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Authors | Peng Zhang, Hong Li, Mo-Li Wu, Xiao-Yan Chen, Qing-You Kong, Xiao-Wei Wang, Yuan Sun, Shu Wen, Jia Liu |
Journal | Journal of neuro-oncology
(J Neurooncol)
Vol. 80
Issue 2
Pg. 123-31
(Nov 2006)
ISSN: 0167-594X [Print] United States |
PMID | 16724266
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antioxidants
- Neoplasm Proteins
- Oligonucleotides, Antisense
- RNA, Neoplasm
- Stilbenes
- Resveratrol
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Topics |
- Antioxidants
(pharmacology)
- Apoptosis
(drug effects)
- Blotting, Western
- Brain Neoplasms
(pathology)
- Cell Cycle
(drug effects)
- Cell Line, Tumor
- Cell Survival
(drug effects)
- Down-Regulation
(drug effects)
- Flow Cytometry
- Fluorescent Antibody Technique
- Genes, myc
(genetics)
- Humans
- Immunohistochemistry
- Medulloblastoma
(pathology)
- Neoplasm Proteins
(biosynthesis)
- Oligonucleotides, Antisense
(genetics)
- RNA, Neoplasm
(biosynthesis, genetics)
- Resveratrol
- Reverse Transcriptase Polymerase Chain Reaction
- S Phase
(physiology)
- Stilbenes
(pharmacology)
- Transfection
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