Anti-inflammatory effect of amurensin H on asthma-like reaction induced by allergen in sensitized mice.

Abstract

AIM:

To explore the anti-inflammatory effects of amurensin H on asthma-like reaction induced by allergen in sensitized mice.

METHODS:

BALB/c mice were sensitized by ovalbumin (OVA, ip) on d 0 and d 14 and challenged with 1% OVA on d 18 to 22. Mice developed airway eosinophilia, mucus hypersecretion, and elevation in cytokine levels. Mice were administered amurensin H orally at the doses of 49, 70, or 100 mg/kg once every day from d 15 to the last day. Bronchoalveolar lavage fluid (BALF) were collected at 24 h and 48 h after the last OVA challenge. Levels of tumor necrosis factor-alpha (TNF-alpha), interleukin 4 (IL-4), interleukin 5 (IL-5), and interleukin 13 (IL-13) in BALF were measured using ELISA method. Differential cell counts of macrophages, lymphocytes, neutrophils and eosinophils were performed in 200 cells per slide (one slide per animal). Lung tissue sections of 6-mum thickness were stained with Mayer's hematoxylin and eosin for assessment of cell infiltration, mucus production, and tissue damage.

RESULTS:

Oral administration of amurensin H significantly inhibited OVA-induced increases in total cell counts, eosinophil counts, and TNF- alpha, IL-4, IL-5 and IL-13 levels in BALF. In addition, amuresin H dramatically decreased OVA-induced lung tissue damage and mucus production.

CONCLUSION:

Amurensin H may have therapeutic potential for the treatment of allergic airway inflammation.

• Authors: Yi-Tang Li, Chun-Suo Yao, Jin-Ye Bai, Mao Lin, Gui-Fang Cheng
• Journal: Acta pharmacologica Sinica (Acta Pharmacol Sin) Vol. 27 Issue 6 Pg. 735-40 (Jun 2006) ISSN: 1671-4083 [Print] United States
• PMID: 16723093 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Anti-Inflammatory Agents
• Benzofurans
• Cytokines
• Interleukin-13
• Interleukin-5
• Stilbenes
• Tumor Necrosis Factor-alpha
• amurensin H
• Interleukin-4
• Ovalbumin

Topics

• Animals
• Anti-Inflammatory Agents (isolation & purification, pharmacology)
• Asthma (chemically induced, drug therapy, metabolism)
• Benzofurans (isolation & purification, pharmacology)
• Cytokines (metabolism)
• Interleukin-13 (metabolism)
• Interleukin-4 (metabolism)
• Interleukin-5 (metabolism)
• Lung (pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Molecular Structure
• Ovalbumin
• Plants, Medicinal (chemistry)
• Stilbenes (isolation & purification, pharmacology)
• Tumor Necrosis Factor-alpha (metabolism)
• Vitis (chemistry)