Abstract |
Quaternary ammonium drugs ( atracurium, bretylium, edrophonium, ipratropium, mivacurium, neostigmine, pancuronium and rocuronium) and herbicides ( difenzoquat, diquat and paraquat) in human whole blood were analysed by LC/MS/MS with positive electrospray ionisation (ESI), following extraction with Bond Elut LRC-CBA cartridges. Internal standards were benzyldimethylphenylammonium chloride monohydrate and ethyl viologen for drug and herbicide analysis, respectively. Ion-pair chromatography used heptafluorobutyric acid (15 mM)-ammonium formate (20 mM) buffer adjusted to pH 3.30 with formic acid and a linear gradient from 5 to 90% methanol run over 18 min. Recoveries ranged from 79.7 to 105.1%, detection limits were between 3.6 and 20.4 ng/ml and the intra- and inter-day precisions were less than 18.6% at a concentration of 10 ng/ml. The method was applied to a case of accidental paraquat poisoning in which the concentration of paraquat in blood was 0.64 mg/l, which is within the range associated with fatal paraquat poisoning.
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Authors | Marinah M Ariffin, Robert A Anderson |
Journal | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
(J Chromatogr B Analyt Technol Biomed Life Sci)
Vol. 842
Issue 2
Pg. 91-7
(Oct 02 2006)
ISSN: 1570-0232 [Print] Netherlands |
PMID | 16716776
(Publication Type: Journal Article)
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Chemical References |
- Androstanols
- Bretylium Compounds
- Herbicides
- Isoquinolines
- Pyrazoles
- Quaternary Ammonium Compounds
- Atracurium
- Neostigmine
- difenzoquat
- Edrophonium
- Mivacurium
- Diquat
- Ipratropium
- Pancuronium
- Paraquat
- bretylium
- Rocuronium
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Topics |
- Androstanols
(blood)
- Atracurium
(blood)
- Bretylium Compounds
(blood)
- Chromatography, Liquid
(methods)
- Diquat
(blood)
- Edrophonium
(blood)
- Herbicides
(blood)
- Humans
- Ipratropium
(blood)
- Isoquinolines
(blood)
- Mivacurium
- Neostigmine
(blood)
- Pancuronium
(blood)
- Paraquat
(blood)
- Pyrazoles
(blood)
- Quaternary Ammonium Compounds
(blood)
- Reproducibility of Results
- Rocuronium
- Spectrometry, Mass, Electrospray Ionization
(methods)
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