Detection of human parvovirus B19 nonstrutural protein DNA by nested-polymerase chain reaction in gravida serum and pregnant tissues.

Abstract

A new nested-polymerase chain reaction (nested-PCR) assay was developed to detect human parvovirus B19 DNA corresponding to the nonstructural protein in clinical specimens in a routine diagnostic laboratory. The sensitivity of this highly specific assay was up to 0.005 fg of B19 DNA. Parvovirus B19 was identified in sera of 20 pregnant women with abnormal pregnant outcome. Among these 20 cases, intrauterine parvovirus infection did exist in 7 pregnant women because parvovirus B19 DNA was detected in the pregnant tissues of them such as placenta tissues, chorionic villi, amniotic fluid, fetal spleen, liver and abdominal fluids.

• Authors: Ting Shen, Yongmei Huang, Fuyuan Qiao, Zengqing Li, Haiyi Liu
• Journal: Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban (J Huazhong Univ Sci Technolog Med Sci) Vol. 26 Issue 1 Pg. 123-6 ( 2006) ISSN: 1672-0733 [Print] China
• PMID: 16711025 (Publication Type: Journal Article)

Chemical References

• DNA, Viral
• NS1 protein, parvovirus
• Viral Nonstructural Proteins

Topics

• Adult
• DNA, Viral (analysis)
• Female
• Humans
• Parvoviridae Infections (virology)
• Parvovirus B19, Human (genetics, isolation & purification)
• Placenta (virology)
• Pregnancy
• Pregnancy Complications, Infectious (diagnosis, virology)
• Viral Nonstructural Proteins (analysis)