Abstract | BACKGROUND: AIM: To identify colonic epithelial cell (CEC)-derived factors that activate CLPFs. METHODS: Primary human CECs and CLPFs were isolated from control mucosa and interleukin 8 ( IL8) of CLPF cultures was quantified by ELISA. Activation of nuclear factor kappaB ( NF-kappaB) was shown, and translocation of NF-kappaB was inhibited by a dominant-negative IkappaB-expressing adenovirus. The major CLPF-activating and IL8 inducing protein was purified using fast-performance liquid chromatography (HiPrep 16/60 Sephacryl S-200 High Resolution Column) and sodium dodecyl sulphate gel electrophoresis. RESULTS: CONCLUSIONS: Using a classical biochemical approach, soluble galectin-3 was identified as a strong activator of CLPFs produced by CEC. Galectin-3 induced NF-kappaB activation and IL8 secretion in these cells and may be a target for future therapeutic approaches to reduce or avoid stricture formation.
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Authors | E Lippert, W Falk, F Bataille, T Kaehne, M Naumann, M Goeke, H Herfarth, J Schoelmerich, G Rogler |
Journal | Gut
(Gut)
Vol. 56
Issue 1
Pg. 43-51
(Jan 2007)
ISSN: 0017-5749 [Print] England |
PMID | 16709662
(Publication Type: Journal Article)
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Chemical References |
- Culture Media, Conditioned
- Galectin 3
- Interleukin-8
- NF-kappa B
- Transcription Factor AP-1
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Topics |
- Adult
- Aged
- Aged, 80 and over
- Caco-2 Cells
- Cell Line
- Cells, Cultured
- Colon
(chemistry)
- Colorectal Neoplasms
(pathology)
- Culture Media, Conditioned
- Diverticulitis
(pathology)
- Epithelial Cells
(chemistry)
- Female
- Fibroblasts
(drug effects)
- Galectin 3
(analysis)
- HT29 Cells
- Humans
- Interleukin-8
(analysis)
- Intestinal Diseases
(pathology)
- Male
- Middle Aged
- Mucous Membrane
(chemistry)
- NF-kappa B
(metabolism)
- Transcription Factor AP-1
(metabolism)
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