HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Heterogeneous nuclear ribonucleoprotein G shows tumor suppressive effect against oral squamous cell carcinoma cells.

AbstractPURPOSE:
Heterogeneous nuclear ribonucleoproteins (hnRNP) are nucleic acid binding proteins involved in RNA processing. We found that hnRNP G is expressed in normal human oral epithelial cells while frequently not found in the cells derived from human oral squamous cell carcinomas (HOSCC). The current study was designed to test the hypothesis that hnRNP G is a tumor suppressor.
EXPERIMENTAL DESIGN:
We investigated the expression levels of hnRNP G protein in normal, precancerous, and malignant oral tissues by in situ immunohistochemistry. In addition, wild-type or mutant hnRNP G was ectopically overexpressed in HOSCC cells and their effects on cellular replication kinetics, colonogenic efficiency, anchorage-independent growth, and in vivo tumorigenicity were determined.
RESULTS:
In situ immunohistochemical staining showed robust presence of hnRNP G in the basal cell layers of normal oral epithelium but the level of its staining was markedly reduced in dysplastic or cancerous tissues. Ectopic expression of wild-type hnRNP G in cancer cells lacking hnRNP G expression or containing mutant hnRNP G resulted in severe retardation of proliferation, reduction of colonogenic efficiency, loss of anchorage-independent growth, and reduction of in vivo tumorigenicity in immunocompromised mice. In addition, hnRNP G overexpression led to up-regulation of the expression of TXNIP, a cell cycle inhibitory gene, and significantly reduced the expression of the genes that promote cellular proliferation, such as EGR1, JUND, JUNB, FOS, FOSL1, ROS, and KIT.
CONCLUSIONS:
These results indicate that hnRNP G is a tumor suppressor against HOSCC but its mechanisms of action remain to be further investigated.
AuthorsKi-Hyuk Shin, Mo K Kang, Reuben H Kim, Russell Christensen, No-Hee Park
JournalClinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 12 Issue 10 Pg. 3222-8 (May 15 2006) ISSN: 1078-0432 [Print] United States
PMID16707624 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References
  • Heterogeneous-Nuclear Ribonucleoproteins
  • RBMX protein, human
Topics
  • Animals
  • Carcinoma, Squamous Cell (genetics, physiopathology)
  • Cell Adhesion
  • Cell Proliferation
  • Cell Transformation, Neoplastic
  • Down-Regulation
  • Gene Expression Profiling
  • Gene Expression Regulation, Neoplastic (drug effects)
  • Heterogeneous-Nuclear Ribonucleoproteins (biosynthesis, genetics, physiology)
  • Humans
  • Immunocompromised Host
  • Immunohistochemistry
  • Mice
  • Mouth Neoplasms (genetics, physiopathology)
  • Precancerous Conditions
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tumor Cells, Cultured
  • Up-Regulation

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: