Low prevalence of transfusion transmitted virus (TTV)-like DNA sequences in cystadenolymphoma and pleomorphic adenoma of the salivary glands.

Abstract

Titers of transfusion transmitted virus (TTV)-like DNA in saliva samples have been reported 100-1,000 times higher than those of the corresponding sera, suggesting viral transmission by saliva droplets. The present study was conducted to determine whether TTV-like DNA sequence elements play a role in the pathogenesis of cystadenolymphoma or pleomorphic adenoma and if the parotid or the submandibular gland is a major source of TTV persistence. Sixty-two archival salivary gland samples (16 cystadenolymphomas, 13 pleomorphic adenomas, and 33 controls) and 23 corresponding saliva samples were examined using a polymerase chain reaction (PCR) for TTV DNA. All PCR products that displayed DNA bands were sequenced. Leder's stain and immunohistochemistry (anti-CD8, anti-CD20, anti-CD45R0, anti-CD68, and anti-Ki67/MiB1) were applied to detect possible changes associated with findings of TTV-like DNA sequences. Tissue displayed TTV-like DNA sequences in 8.1% (5/62; saliva: 47.8%, 11/23). Tissue that contained TTV-like DNA sequences was histologically indistinguishable from samples lacking such DNA. TTV appears to be only a bystander in cystadenolymphoma, pleomorphic adenoma, and other salivary gland affections. Neither of the glands seems to be a major source of TTV persistence.

• Authors: Mathias Wagner, Jens P Klussmann, Olaf Dirsch, Vivienne A Rose, Orlando Guntinas-Lichius, Dirk Theegarten, Holger Sudhoff, Roland Linder
• Journal: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery (Eur Arch Otorhinolaryngol) Vol. 263 Issue 8 Pg. 759-63 (Aug 2006) ISSN: 0937-4477 [Print] Germany
• PMID: 16703387 (Publication Type: Journal Article)

Chemical References

• DNA, Viral

Topics

• Adenolymphoma (pathology, virology)
• Adenoma, Pleomorphic (pathology, virology)
• Adult
• Aged
• Aged, 80 and over
• Base Sequence
• Case-Control Studies
• DNA, Viral (analysis)
• Female
• Humans
• Immunohistochemistry
• Male
• Middle Aged
• Molecular Sequence Data
• Parotid Gland (pathology, virology)
• Polymerase Chain Reaction
• Prevalence
• Saliva (virology)
• Salivary Gland Neoplasms (pathology, virology)
• Sequence Analysis, DNA
• Submandibular Gland (pathology, virology)
• Torque teno virus (genetics, isolation & purification)