Abstract |
To detect the expression of telomerase subunits (human telomerase reverse transcriptase, human telomerase associated protein 1 and human telomerase RNA) in gastric cancer and to examine the role that different telomerase subunits play in the gastric carcinogenesis, reverse transcription-polymerase chain reaction (RT-PCR) was used to detect telomerase subunits messenger RNA in 24 samples of gastric cancer and corresponding non-cancerous tissue. The results showed that the positive rate of hTERT mRNA from gastric cancer and corresponding non-cancerous tissues was 100% and 25%, respectively. The former was significantly higher than the latter (chi2 = 26.4, P < 0.01). The positive rate of hTEP1 mRNA from gastric cancer and corresponding non-cancerous tissues was 100% and 91.7%, respectively and no significant difference was found between them (chi2 = 2.1, P > 0.05). The positive rates of hTR for gastric cancer and corresponding non-cancerous tissues were both 100% and no significant difference existed between them. It is concluded that in contrast to hTEP1 and hTR, the up-regulation of hTERT mRNA expression may play a more important role in the development of gastric cancer.
|
Authors | Fenghua Chen, Lihua Hu, Yirong Li, Lin Wang |
Journal | Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban
(J Huazhong Univ Sci Technolog Med Sci)
Vol. 25
Issue 6
Pg. 741-3
( 2005)
ISSN: 1672-0733 [Print] China |
PMID | 16696344
(Publication Type: Journal Article)
|
Chemical References |
- Carrier Proteins
- RNA, Messenger
- RNA-Binding Proteins
- TEP1 protein, human
- telomerase RNA
- RNA
- TERT protein, human
- Telomerase
|
Topics |
- Adult
- Aged
- Carrier Proteins
(biosynthesis, genetics)
- Female
- Humans
- Male
- Middle Aged
- RNA
(biosynthesis, genetics)
- RNA, Messenger
(biosynthesis, genetics)
- RNA-Binding Proteins
- Reverse Transcriptase Polymerase Chain Reaction
- Stomach Neoplasms
(metabolism)
- Telomerase
(biosynthesis, genetics)
|