Abstract |
By utilizing a novel combinatorial method of a Laser Microdissection System and Western blot analysis, we demonstrate that a distinct isoform of abnormally phosphorylated tau (69 kDa, Tau 69) predominantly aggregated in laser-microdissected Pick bodies (PBs) in sporadic Pick's disease. By contrast, tau migrated as two major bands of 60 and 64 kDa (Tau 60 and 64) in total brain homogenates as previously reported. Comparative immunohistochemical analysis with anti-4-repeat antibody revealed that a major component of the abnormally phosphorylated tau in these PBs was 3-repeat tau (3R-tau). Whether 29 amino acid repeat encoded by exons 2 and 3 in the Tau 69 might accelerate the formation of PBs remains to be further investigated. Such a combination of morphological and biochemical techniques significantly complements the existing histopathological methods.
|
Authors | Takuya Ohkubo, Yuji Sakasegawa, Hiroyuki Toda, Hitaru Kishida, Kunimasa Arima, Mitsunori Yamada, Hitoshi Takahashi, Hidehiro Mizusawa, Naomi S Hachiya, Kiyotoshi Kaneko |
Journal | Amyloid : the international journal of experimental and clinical investigation : the official journal of the International Society of Amyloidosis
(Amyloid)
Vol. 13
Issue 1
Pg. 1-5
(Mar 2006)
ISSN: 1350-6129 [Print] England |
PMID | 16690493
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Protein Isoforms
- tau Proteins
|
Topics |
- Aged
- Female
- Humans
- Inclusion Bodies
(chemistry, metabolism)
- Lasers
- Microdissection
- Middle Aged
- Phosphorylation
- Pick Disease of the Brain
(metabolism, pathology)
- Protein Isoforms
(chemistry, metabolism)
- Repetitive Sequences, Amino Acid
- tau Proteins
(chemistry, metabolism)
|