Recent studies of animal models have suggested that an increase in the number of T cells due to both peripheral expansion and increased thymic T cell output plays a key role in the regulation of bone loss after
ovariectomy. Osteoclastogenic
cytokines which are either produced by T cells or activate T cells have also been implicated in ovx induced bone loss. Among them are
TNF alpha and
IL-7. The present study investigates the role of
thymectomy (THX) and
IL-7 in bone metabolism in humans. We studied T cells subsets,
cytokine production and bone metabolism in 13 women thymectomized for
Myasthenia gravis as compared to healthy controls. Our data demonstrate that the number of CD4+ and TNF-producing T cells is lower in THX women as compared to euthymic controls. However in THX women the residual T cells produce higher levels of
IL-7 and RANKL. Furthermore, flow cytometry shows that
IL-7 is produced by T and B cells. Serum levels of
TNF alpha were unaffected by THX and both serum
TNF alpha and the RANKL/OPG correlated inversely with BMD. There were no differences in bone turnover and bone mineral density between THX women and the controls. These data suggest that THX decreases the number of TNF-producing CD4+ T cells but does not alters serum TNF levels. The RANKL/OPG ratio and indices of bone metabolisms are also not affected by THX, although THX increases the levels of
IL-7 and RANKL. Further studies are needed to clarify the role of thymus in bone metabolism and osteoclastogenesis in postmenopausal women.