Functional analysis of the CPS I upstream sequences with a cat assay.

Abstract

Expression plasmids (pKCPS-CAT) containing carbamyl phosphate synthetase (CPS I) upstream sequences of different lengths were constructed, and the function and characteristics of the sequences were studied with the CAT assay. Results showed that the CPS I upstream sequences exerted highly tissue-specific control on CPS I gene expression, and the -113 approximately -38 bp region relative to the cap site was found to be indispensable for CPS I gene transcription. The -1700 approximately -161 bp region contains sequences which confer an enhancing effect on CPS I gene transcription. Dexamethasone and thioproline (a differentiation inducer) showed enhancing effects on CPS I gene transcription in hepatoma cells. These results would have significance in studies on the gene regulation of CPS I associated with the mechanism of hepatocyte differentiation and carcinogenesis.

• Authors: B Niu, Z Wang, Y Yu, M Wang, S Zeng, S Li
• Journal: Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih (Chin Med Sci J) Vol. 6 Issue 4 Pg. 208-11 (Dec 1991) ISSN: 1001-9294 [Print] China
• PMID: 1667483 (Publication Type: Journal Article)

Chemical References

• Antineoplastic Agents
• Thiazoles
• Thiazolidines
• thiazolidine-4-carboxylic acid
• Carbamoyl-Phosphate Synthase (Ammonia)

Topics

• Antineoplastic Agents (pharmacology)
• Carbamoyl-Phosphate Synthase (Ammonia) (genetics)
• Carcinoma, Hepatocellular (pathology)
• Gene Expression (drug effects)
• Humans
• Liver Neoplasms (pathology)
• Plasmids
• Regulatory Sequences, Nucleic Acid (physiology)
• Thiazoles (pharmacology)
• Thiazolidines
• Transcription, Genetic
• Transfection
• Tumor Cells, Cultured