[Mutations of DNA gyrase and topoisomerase IV in clinical isolates of fluoroquinolone-resistant Proteus mirabilis].

Abstract

The presence of fluoroquinolone resistance-associated mutations within the quinolone resistance-determining region of DNA gyrase and topoisomerase IV was investigated genetically in clinical isolates of Proteus mirabilis recovered from patients with urinay tract infections. Two isolates of fluoroquinolone-resistant P. mirabilis possessed the mutations in GyrA (Ser-83 --> Arg or Ile), GyrB (Ser-464 --> Tyr or Phe) and ParC (Ser-80 --> Ile). A novel mutation with Glu-87 --> Lys in GyrA, where suggested to be responsible for fluoroquinolone resistance, was identified. These results demonstrate that the presence of an additional mutation at Glu-87 in GyrA may contribute to high-level fluoroquinolone resistance, too.

• Authors: Ryoichi Saito, Kenya Sato, Wakako Kumita, Natsuko Inami, Hiroyuki Nishiyama, Noboru Okamura, Kyoji Moriya, Kazuhiko Koike
• Journal: The Japanese journal of antibiotics (Jpn J Antibiot) Vol. 59 Issue 1 Pg. 41-3 (Feb 2006) ISSN: 0368-2781 [Print] Japan
• PMID: 16673582 (Publication Type: Journal Article)

Chemical References

• Fluoroquinolones
• DNA Topoisomerase IV
• DNA Gyrase

Topics

• DNA Gyrase (genetics)
• DNA Topoisomerase IV (genetics)
• Drug Resistance, Bacterial
• Fluoroquinolones (pharmacology)
• Humans
• Mutation
• Proteus mirabilis (drug effects, enzymology, genetics, isolation & purification)
• Urinary Tract Infections (microbiology)