Abstract |
Previous investigations from our laboratory show that up-regulation of neuronal nitric oxide synthase (NOS) following spinal cord injury (SCI) is injurious to the cord. Antiserum to dynorphin A (1-17) induces marked neuroprotection in our model of SCI, indicating an interaction between dynorphin and NOS regulation. The present investigation was undertaken to find out whether topical application of dynorphin A (1-17) antiserum has some influence on neuronal NOS up-regulation in the traumatized spinal cord. SCI was produced in anesthetized animals by making a unilateral incision into the right dorsal horn of the T10-11 segments. The antiserum to dynorphin A (1-17) was applied (1 : 20, 20 microL in 10 seconds) 5 minutes after trauma over the injured spinal cord and the rats were allowed to survive 5 hours after SCI. Topical application of dynorphin A (1-17) antiserum significantly attenuated neuronal NOS up-regulation in the adjacent T9 and T12 segments. In the antiserum-treated group, spinal cord edema and cell injury were also less marked. These observations provide new evidence that the opioid active peptide dynorphin A may be involved in the mechanisms underlying NOS regulation in the spinal cord after injury, and confirms our hypothesis that up-regulation of neuronal NOS is injurious to the cord.
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Authors | H S Sharma, F Nyberg, T Gordh, P Alm |
Journal | Acta neurochirurgica. Supplement
(Acta Neurochir Suppl)
Vol. 96
Pg. 309-15
( 2006)
ISSN: 0065-1419 [Print] Austria |
PMID | 16671477
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antibodies
- Neuroprotective Agents
- Dynorphins
- Nitric Oxide Synthase Type I
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Topics |
- Animals
- Antibodies
(administration & dosage, immunology)
- Dynorphins
(immunology)
- Edema
(etiology, immunology, prevention & control)
- Male
- Neuroprotective Agents
(administration & dosage)
- Nitric Oxide Synthase Type I
(immunology)
- Rats
- Rats, Sprague-Dawley
- Spinal Cord Injuries
(complications, drug therapy, immunology)
- Treatment Outcome
- Up-Regulation
(drug effects)
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